Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2555864..2557452 Replicon chromosome
Accession NZ_CP113503
Organism Escherichia coli strain 16843

Toxin (Protein)


Gene name hipA Uniprot ID A0A192ENQ5
Locus tag OWO50_RS12080 Protein ID WP_001125432.1
Coordinates 2556130..2557452 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag OWO50_RS12075 Protein ID WP_001304368.1
Coordinates 2555864..2556130 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OWO50_RS12060 (2552232) 2552232..2553683 + 1452 WP_000854642.1 tagaturonate reductase -
OWO50_RS12065 (2553880) 2553880..2554794 + 915 WP_001298637.1 bestrophin family protein -
OWO50_RS12070 (2554798) 2554798..2555556 - 759 WP_001286538.1 trans-aconitate 2-methyltransferase -
OWO50_RS12075 (2555864) 2555864..2556130 + 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
OWO50_RS12080 (2556130) 2556130..2557452 + 1323 WP_001125432.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
OWO50_RS12085 (2557628) 2557628..2558320 - 693 WP_000382466.1 hypothetical protein -
OWO50_RS12090 (2558332) 2558332..2558607 - 276 WP_000948310.1 hypothetical protein -
OWO50_RS12095 (2558609) 2558609..2558749 - 141 WP_001335097.1 hypothetical protein -
OWO50_RS12100 (2558865) 2558865..2559047 - 183 WP_001335098.1 hypothetical protein -
OWO50_RS12105 (2559217) 2559217..2560269 + 1053 WP_000568119.1 phage/plasmid replication protein, II/X family -
OWO50_RS12110 (2560281) 2560281..2560598 + 318 WP_000073834.1 DNA-binding protein -
OWO50_RS12115 (2560802) 2560802..2561044 + 243 WP_000699011.1 phage capsid protein -
OWO50_RS12120 (2561091) 2561091..2562371 + 1281 WP_000823306.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49297.02 Da        Isoelectric Point: 8.6972

>T265824 WP_001125432.1 NZ_CP113503:2556130-2557452 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT265824 WP_001304368.1 NZ_CP113503:2555864-2556130 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192ENQ5

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References