Detailed information of TA system TA026582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2189457..2189986
• Replicon: chromosome
• Accession: NC_017333
• Organism: Staphylococcus aureus subsp. aureus ST398

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAPIG_RS11065
• Protein ID: WP_000621175.1
• Coordinates: 2189457..2189819 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAPIG_RS11070
• Protein ID: WP_000948331.1
• Coordinates: 2189816..2189986 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAPIG_RS11040	2186435..2187205	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SAPIG_RS11045	2187180..2187659	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
SAPIG_RS11050	2187661..2187987	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAPIG_RS11055	2188106..2189107	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAPIG_RS11065	2189457..2189819	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAPIG_RS11070	2189816..2189986	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAPIG_RS11075	2190071..2191219	-	1149	WP_001281139.1	alanine racemase	-
SAPIG_RS11080	2191285..2191644	-	360	WP_000581197.1	holo-ACP synthase	-
SAPIG_RS11085	2191648..2192139	-	492	WP_001286980.1	PH domain-containing protein	-
SAPIG_RS11090	2192132..2193709	-	1578	WP_001294650.1	PH domain-containing protein	-
SAPIG_RS11095	2193702..2194181	-	480	WP_001287081.1	hypothetical protein	-
SAPIG_RS11100	2194390..2194950	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T26582 WP_000621175.1 NC_017333:c2189819-2189457 [Staphylococcus aureus subsp. aureus ST398]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T26582 NC_017333:c2189819-2189457 [Staphylococcus aureus subsp. aureus ST398]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT26582 WP_000948331.1 NC_017333:c2189986-2189816 [Staphylococcus aureus subsp. aureus ST398]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT26582 NC_017333:c2189986-2189816 [Staphylococcus aureus subsp. aureus ST398]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1