Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1191883..1192466 Replicon chromosome
Accession NZ_CP113503
Organism Escherichia coli strain 16843

Toxin (Protein)


Gene name mazF Uniprot ID S1PVD8
Locus tag OWO50_RS05710 Protein ID WP_000254749.1
Coordinates 1192131..1192466 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag OWO50_RS05705 Protein ID WP_000581937.1
Coordinates 1191883..1192131 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OWO50_RS05695 (1188222) 1188222..1189523 + 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
OWO50_RS05700 (1189571) 1189571..1191805 + 2235 WP_000226797.1 GTP diphosphokinase -
OWO50_RS05705 (1191883) 1191883..1192131 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
OWO50_RS05710 (1192131) 1192131..1192466 + 336 WP_000254749.1 endoribonuclease MazF Toxin
OWO50_RS05715 (1192538) 1192538..1193329 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
OWO50_RS05720 (1193557) 1193557..1195194 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
OWO50_RS05725 (1195282) 1195282..1196580 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12137.06 Da        Isoelectric Point: 8.7218

>T265817 WP_000254749.1 NZ_CP113503:1192131-1192466 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT265817 WP_000581937.1 NZ_CP113503:1191883-1192131 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PVD8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References