Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4718008..4718505 Replicon chromosome
Accession NZ_CP113493
Organism Escherichia coli strain GTEN_97

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag E5R32_RS22900 Protein ID WP_001173343.1
Coordinates 4718008..4718181 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag E5R32_RS22905 Protein ID WP_000398619.1
Coordinates 4718209..4718505 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5R32_RS22890 (4714678) 4714678..4716135 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
E5R32_RS22895 (4716372) 4716372..4717889 + 1518 WP_001550472.1 lysine--tRNA ligase -
E5R32_RS22900 (4718008) 4718008..4718181 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
E5R32_RS22905 (4718209) 4718209..4718505 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
E5R32_RS22910 (4718734) 4718734..4719006 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
E5R32_RS22915 (4719018) 4719018..4719248 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
E5R32_RS22920 (4719428) 4719428..4721059 + 1632 WP_001216477.1 sensor histidine kinase -
E5R32_RS22925 (4721056) 4721056..4721775 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4672661..4718505 45844


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T265800 WP_001173343.1 NZ_CP113493:c4718181-4718008 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT265800 WP_000398619.1 NZ_CP113493:c4718505-4718209 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References