Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2480414..2481198 Replicon chromosome
Accession NZ_CP113493
Organism Escherichia coli strain GTEN_97

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag E5R32_RS11950 Protein ID WP_000613626.1
Coordinates 2480414..2480908 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag E5R32_RS11955 Protein ID WP_001110447.1
Coordinates 2480905..2481198 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5R32_RS11930 (2475607) 2475607..2476704 + 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -
E5R32_RS11935 (2476704) 2476704..2477645 + 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
E5R32_RS11940 (2477711) 2477711..2479354 + 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
E5R32_RS11945 (2479366) 2479366..2480319 + 954 WP_001212768.1 flagellar hook-associated protein FlgL -
E5R32_RS11950 (2480414) 2480414..2480908 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
E5R32_RS11955 (2480905) 2480905..2481198 - 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
E5R32_RS11960 (2481331) 2481331..2484516 - 3186 WP_001550951.1 ribonuclease E -
E5R32_RS11965 (2485089) 2485089..2486048 + 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T265787 WP_000613626.1 NZ_CP113493:c2480908-2480414 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT265787 WP_001110447.1 NZ_CP113493:c2481198-2480905 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References