Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1212645..1213221 Replicon chromosome
Accession NZ_CP113493
Organism Escherichia coli strain GTEN_97

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag E5R32_RS05985 Protein ID WP_001297643.1
Coordinates 1212645..1212932 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag E5R32_RS05990 Protein ID WP_000063160.1
Coordinates 1212919..1213221 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5R32_RS05970 (1208882) 1208882..1210162 - 1281 WP_001037390.1 DUF445 domain-containing protein -
E5R32_RS05975 (1210203) 1210203..1211435 - 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
E5R32_RS05980 (1211750) 1211750..1212446 + 697 Protein_1175 IS1 family transposase -
E5R32_RS05985 (1212645) 1212645..1212932 + 288 WP_001297643.1 BrnT family toxin Toxin
E5R32_RS05990 (1212919) 1212919..1213221 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
E5R32_RS05995 (1213255) 1213255..1214211 - 957 WP_001297640.1 GTPase -
E5R32_RS06000 (1214222) 1214222..1214425 - 204 WP_000467859.1 YbdD/YjiX family protein -
E5R32_RS06005 (1214475) 1214475..1216625 - 2151 WP_001340937.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1212237..1212446 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T265782 WP_001297643.1 NZ_CP113493:1212645-1212932 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT265782 WP_000063160.1 NZ_CP113493:1212919-1213221 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References