Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3575214..3575790 Replicon chromosome
Accession NZ_CP113486
Organism Escherichia coli strain GTEN_23

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag FG550_RS17220 Protein ID WP_001297643.1
Coordinates 3575214..3575501 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag FG550_RS17225 Protein ID WP_000063160.1
Coordinates 3575488..3575790 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FG550_RS17205 (3571452) 3571452..3572732 - 1281 WP_001037390.1 DUF445 domain-containing protein -
FG550_RS17210 (3572773) 3572773..3574005 - 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
FG550_RS17215 (3574319) 3574319..3575015 + 697 Protein_3359 IS1 family transposase -
FG550_RS17220 (3575214) 3575214..3575501 + 288 WP_001297643.1 BrnT family toxin Toxin
FG550_RS17225 (3575488) 3575488..3575790 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
FG550_RS17230 (3575824) 3575824..3576780 - 957 WP_001297640.1 GTPase -
FG550_RS17235 (3576791) 3576791..3576994 - 204 WP_000467859.1 YbdD/YjiX family protein -
FG550_RS17240 (3577044) 3577044..3579194 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3574806..3575015 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T265759 WP_001297643.1 NZ_CP113486:3575214-3575501 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT265759 WP_000063160.1 NZ_CP113486:3575488-3575790 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References