Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 245206..245848 Replicon chromosome
Accession NZ_CP113428
Organism Bacillus cereus strain NW6

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag OU819_RS01385 Protein ID WP_000635963.1
Coordinates 245498..245848 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag OU819_RS01380 Protein ID WP_000004570.1
Coordinates 245206..245493 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OU819_RS01355 (OU819_01355) 240522..241484 + 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -
OU819_RS01360 (OU819_01360) 241477..242049 - 573 WP_088015323.1 rhomboid family intramembrane serine protease -
OU819_RS01365 (OU819_01365) 242142..242501 + 360 WP_000635040.1 holo-ACP synthase -
OU819_RS01370 (OU819_01370) 242658..243608 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
OU819_RS01375 (OU819_01375) 243727..244896 + 1170 WP_000390600.1 alanine racemase -
OU819_RS01380 (OU819_01380) 245206..245493 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
OU819_RS01385 (OU819_01385) 245498..245848 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
OU819_RS01390 (OU819_01390) 245916..248084 + 2169 WP_000426225.1 Tex family protein -
OU819_RS01395 (OU819_01395) 248142..248258 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
OU819_RS01400 (OU819_01400) 248454..248912 + 459 WP_078437659.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T265704 WP_000635963.1 NZ_CP113428:245498-245848 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT265704 WP_000004570.1 NZ_CP113428:245206-245493 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References