Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1241204..1242121 Replicon chromosome
Accession NZ_CP113418
Organism Bacillus amyloliquefaciens strain WH1

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag OU987_RS06500 Protein ID WP_007407256.1
Coordinates 1241375..1242121 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag OU987_RS06495 Protein ID WP_003154807.1
Coordinates 1241204..1241374 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OU987_RS06455 (OU987_06455) 1236437..1238059 + 1623 WP_032874601.1 pyocin knob domain-containing protein -
OU987_RS06460 (OU987_06460) 1238072..1238443 + 372 WP_032874603.1 XkdW family protein -
OU987_RS06465 (OU987_06465) 1238448..1238645 + 198 WP_032874605.1 XkdX family protein -
OU987_RS06470 (OU987_06470) 1238702..1239463 + 762 WP_032874607.1 hypothetical protein -
OU987_RS06475 (OU987_06475) 1239515..1239778 + 264 WP_003154815.1 hemolysin XhlA family protein -
OU987_RS06480 (OU987_06480) 1239792..1240055 + 264 WP_003154813.1 phage holin -
OU987_RS06485 (OU987_06485) 1240069..1240947 + 879 WP_032874609.1 N-acetylmuramoyl-L-alanine amidase -
OU987_RS06490 (OU987_06490) 1240982..1241107 - 126 WP_003154809.1 hypothetical protein -
OU987_RS06495 (OU987_06495) 1241204..1241374 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
OU987_RS06500 (OU987_06500) 1241375..1242121 - 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
OU987_RS06505 (OU987_06505) 1242226..1243224 - 999 WP_032874611.1 inorganic phosphate transporter -
OU987_RS06510 (OU987_06510) 1243237..1243854 - 618 WP_032874614.1 DUF47 domain-containing protein -
OU987_RS06515 (OU987_06515) 1244140..1245456 - 1317 WP_032874616.1 amino acid permease -
OU987_RS06520 (OU987_06520) 1245778..1246728 + 951 WP_032874618.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1197450..1299973 102523


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T265703 WP_007407256.1 NZ_CP113418:c1242121-1241375 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT265703 WP_003154807.1 NZ_CP113418:c1241374-1241204 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2HQ15

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References