Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1319281..1320197 Replicon chromosome
Accession NZ_CP113256
Organism Bacillus subtilis strain K1

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag ORP33_RS06930 Protein ID WP_003244695.1
Coordinates 1319451..1320197 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag ORP33_RS06925 Protein ID WP_003232646.1
Coordinates 1319281..1319451 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORP33_RS06890 (1316141) 1316141..1316470 + 330 WP_080529228.1 XkdW family protein -
ORP33_RS06895 (1316467) 1316467..1316631 + 165 WP_047182493.1 XkdX family protein -
ORP33_RS06900 (1316678) 1316678..1317517 + 840 WP_029317677.1 phage-like element PBSX protein XepA -
ORP33_RS06905 (1317570) 1317570..1317839 + 270 WP_003232655.1 hemolysin XhlA family protein -
ORP33_RS06910 (1317852) 1317852..1318115 + 264 WP_029317678.1 phage holin -
ORP33_RS06915 (1318128) 1318128..1319021 + 894 WP_029317679.1 N-acetylmuramoyl-L-alanine amidase -
ORP33_RS06920 (1319058) 1319058..1319195 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
ORP33_RS06925 (1319281) 1319281..1319451 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
ORP33_RS06930 (1319451) 1319451..1320197 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
ORP33_RS06935 (1320307) 1320307..1321308 - 1002 WP_003232642.1 inorganic phosphate transporter -
ORP33_RS06940 (1321321) 1321321..1321938 - 618 WP_003218470.1 DUF47 domain-containing protein -
ORP33_RS06945 (1322214) 1322214..1323530 - 1317 WP_080529229.1 serine/threonine exchanger -
ORP33_RS06950 (1323914) 1323914..1324864 + 951 WP_225721960.1 ring-cleaving dioxygenase -
ORP33_RS06955 (1324973) 1324973..1325068 + 96 Protein_1306 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1285238..1340628 55390


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T265661 WP_003244695.1 NZ_CP113256:c1320197-1319451 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT265661 WP_003232646.1 NZ_CP113256:c1319451-1319281 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References