Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 513288..513924 Replicon chromosome
Accession NZ_CP113256
Organism Bacillus subtilis strain K1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ORP33_RS02625 Protein ID WP_003156187.1
Coordinates 513574..513924 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag ORP33_RS02620 Protein ID WP_003225183.1
Coordinates 513288..513569 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORP33_RS02600 (509648) 509648..510247 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
ORP33_RS02605 (510342) 510342..510707 + 366 WP_015252768.1 holo-ACP synthase -
ORP33_RS02610 (510873) 510873..511889 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
ORP33_RS02615 (512003) 512003..513172 + 1170 WP_015252766.1 alanine racemase -
ORP33_RS02620 (513288) 513288..513569 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ORP33_RS02625 (513574) 513574..513924 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ORP33_RS02630 (514039) 514039..514863 + 825 WP_015252765.1 RsbT co-antagonist protein RsbRA -
ORP33_RS02635 (514868) 514868..515233 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
ORP33_RS02640 (515237) 515237..515638 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
ORP33_RS02645 (515650) 515650..516657 + 1008 WP_015252763.1 phosphoserine phosphatase RsbU -
ORP33_RS02650 (516719) 516719..517048 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
ORP33_RS02655 (517045) 517045..517527 + 483 WP_003234299.1 anti-sigma B factor RsbW -
ORP33_RS02660 (517493) 517493..518281 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
ORP33_RS02665 (518281) 518281..518880 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T265660 WP_003156187.1 NZ_CP113256:513574-513924 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT265660 WP_003225183.1 NZ_CP113256:513288-513569 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References