265659

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 5323..5924 Replicon plasmid plas1
Accession NZ_CP113251
Organism Levilactobacillus brevis strain SC013

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag OUY26_RS12235 Protein ID WP_001748110.1
Coordinates 5323..5667 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag OUY26_RS12240 Protein ID WP_001748109.1
Coordinates 5661..5924 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OUY26_RS12210 (OUY26_12210) 373..1527 + 1155 WP_267925647.1 cation:proton antiporter -
OUY26_RS12215 (OUY26_12215) 1659..2434 + 776 WP_267925648.1 IS5-like element ISLpl3 family transposase -
OUY26_RS12220 (OUY26_12220) 2505..4073 + 1569 WP_267925650.1 ClC family H(+)/Cl(-) exchange transporter -
OUY26_RS12225 (OUY26_12225) 4179..4268 + 90 Protein_3 IS30 family transposase -
OUY26_RS12230 (OUY26_12230) 4310..4777 - 468 WP_003587210.1 DNA starvation/stationary phase protection protein -
OUY26_RS12235 (OUY26_12235) 5323..5667 - 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OUY26_RS12240 (OUY26_12240) 5661..5924 - 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
OUY26_RS12245 (OUY26_12245) 6010..6597 + 588 WP_267925651.1 site-specific integrase -
OUY26_RS12250 (OUY26_12250) 6826..7053 + 228 WP_267925654.1 hypothetical protein -
OUY26_RS12255 (OUY26_12255) 7076..7354 + 279 WP_015474680.1 hypothetical protein -
OUY26_RS12260 (OUY26_12260) 7344..7850 - 507 Protein_10 hypothetical protein -
OUY26_RS12265 (OUY26_12265) 7840..8121 - 282 WP_014564139.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
OUY26_RS12270 (OUY26_12270) 8400..8531 - 132 WP_267925652.1 hypothetical protein -
OUY26_RS12275 (OUY26_12275) 9020..9412 - 393 WP_082265502.1 hypothetical protein -
OUY26_RS12280 (OUY26_12280) 9607..9804 - 198 WP_267925653.1 hypothetical protein -
OUY26_RS12285 (OUY26_12285) 9984..10262 - 279 WP_015474677.1 BRCT domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..21989 21989


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T265659 WP_001748110.1 NZ_CP113251:c5667-5323 [Levilactobacillus brevis]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT265659 WP_001748109.1 NZ_CP113251:c5924-5661 [Levilactobacillus brevis]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References