Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 163161..163897 Replicon plasmid pSCLC3-1_3
Accession NZ_CP113201
Organism Klebsiella pneumoniae strain SCLC3-1

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag OT487_RS28690 Protein ID WP_003026803.1
Coordinates 163415..163897 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag OT487_RS28685 Protein ID WP_003026799.1
Coordinates 163161..163427 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OT487_RS28645 (OT487_28645) 158390..158752 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
OT487_RS28650 (OT487_28650) 158802..159152 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
OT487_RS28655 (OT487_28655) 159510..159758 + 249 WP_001568018.1 hypothetical protein -
OT487_RS28660 (OT487_28660) 159755..160327 + 573 WP_001568017.1 hypothetical protein -
OT487_RS28665 (OT487_28665) 160358..160852 + 495 WP_001568016.1 hypothetical protein -
OT487_RS28670 (OT487_28670) 161085..161435 - 351 WP_167878626.1 hypothetical protein -
OT487_RS28675 (OT487_28675) 161887..162435 + 549 WP_001567366.1 thioredoxin fold domain-containing protein -
OT487_RS28680 (OT487_28680) 162482..162916 + 435 WP_001567367.1 cell envelope integrity protein TolA -
OT487_RS28685 (OT487_28685) 163161..163427 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
OT487_RS28690 (OT487_28690) 163415..163897 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
OT487_RS28695 (OT487_28695) 164635..165879 + 1245 WP_124053351.1 hypothetical protein -
OT487_RS28700 (OT487_28700) 165872..166627 + 756 WP_100757158.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA2 / blaTEM-1A / floR / aph(3')-Ia / sul1 / qacE - 1..211625 211625


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T265574 WP_003026803.1 NZ_CP113201:163415-163897 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT265574 WP_003026799.1 NZ_CP113201:163161-163427 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References