Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4558259..4559069 Replicon chromosome
Accession NZ_CP113197
Organism Klebsiella pneumoniae strain SCLC3-1

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag OT487_RS22485 Protein ID WP_004178461.1
Coordinates 4558259..4558792 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag OT487_RS22490 Protein ID WP_002887278.1
Coordinates 4558803..4559069 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OT487_RS22480 (4557090) 4557090..4558211 + 1122 WP_004186850.1 cupin domain-containing protein -
OT487_RS22485 (4558259) 4558259..4558792 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
OT487_RS22490 (4558803) 4558803..4559069 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
OT487_RS22495 (4559172) 4559172..4560605 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
OT487_RS22500 (4560595) 4560595..4561278 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
OT487_RS22505 (4561451) 4561451..4562836 + 1386 WP_064152126.1 efflux transporter outer membrane subunit -
OT487_RS22510 (4562854) 4562854..4563198 + 345 WP_064152125.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T265567 WP_004178461.1 NZ_CP113197:c4558792-4558259 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT265567 WP_002887278.1 NZ_CP113197:c4559069-4558803 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References