Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5148734..5149359 Replicon chromosome
Accession NZ_CP113192
Organism Klebsiella pneumoniae strain SXC4-2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag OT483_RS25205 Protein ID WP_002882817.1
Coordinates 5148734..5149117 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OT483_RS25210 Protein ID WP_004150355.1
Coordinates 5149117..5149359 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OT483_RS25190 (5146100) 5146100..5147002 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
OT483_RS25195 (5146999) 5146999..5147634 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
OT483_RS25200 (5147631) 5147631..5148560 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OT483_RS25205 (5148734) 5148734..5149117 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
OT483_RS25210 (5149117) 5149117..5149359 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OT483_RS25215 (5149564) 5149564..5150481 + 918 WP_004178029.1 alpha/beta hydrolase -
OT483_RS25220 (5150495) 5150495..5151436 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
OT483_RS25225 (5151481) 5151481..5151918 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
OT483_RS25230 (5151915) 5151915..5152775 - 861 WP_004146232.1 virulence factor BrkB family protein -
OT483_RS25235 (5152769) 5152769..5153368 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T265554 WP_002882817.1 NZ_CP113192:c5149117-5148734 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT265554 WP_004150355.1 NZ_CP113192:c5149359-5149117 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References