Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4556497..4557307 Replicon chromosome
Accession NZ_CP113192
Organism Klebsiella pneumoniae strain SXC4-2

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag OT483_RS22415 Protein ID WP_004178461.1
Coordinates 4556497..4557030 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag OT483_RS22420 Protein ID WP_002887278.1
Coordinates 4557041..4557307 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OT483_RS22410 (4555328) 4555328..4556449 + 1122 WP_009309849.1 cupin domain-containing protein -
OT483_RS22415 (4556497) 4556497..4557030 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
OT483_RS22420 (4557041) 4557041..4557307 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
OT483_RS22425 (4557410) 4557410..4558843 - 1434 WP_023280821.1 Cu(+)/Ag(+) sensor histidine kinase -
OT483_RS22430 (4558833) 4558833..4559516 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
OT483_RS22435 (4559689) 4559689..4561074 + 1386 WP_102024742.1 efflux transporter outer membrane subunit -
OT483_RS22440 (4561092) 4561092..4561436 + 345 WP_021462623.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T265551 WP_004178461.1 NZ_CP113192:c4557030-4556497 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT265551 WP_002887278.1 NZ_CP113192:c4557307-4557041 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References