Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5243987..5244612 Replicon chromosome
Accession NZ_CP113191
Organism Klebsiella pneumoniae strain SYCC2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag OT482_RS28510 Protein ID WP_002882817.1
Coordinates 5244229..5244612 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OT482_RS28505 Protein ID WP_004150355.1
Coordinates 5243987..5244229 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OT482_RS28480 (5239978) 5239978..5240577 + 600 WP_004151865.1 glucose-1-phosphatase -
OT482_RS28485 (5240571) 5240571..5241431 + 861 WP_004146232.1 virulence factor BrkB family protein -
OT482_RS28490 (5241428) 5241428..5241865 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
OT482_RS28495 (5241910) 5241910..5242851 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
OT482_RS28500 (5242865) 5242865..5243782 - 918 WP_004181612.1 alpha/beta hydrolase -
OT482_RS28505 (5243987) 5243987..5244229 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OT482_RS28510 (5244229) 5244229..5244612 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
OT482_RS28515 (5244786) 5244786..5245715 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OT482_RS28520 (5245712) 5245712..5246347 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
OT482_RS28525 (5246344) 5246344..5247246 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T265540 WP_002882817.1 NZ_CP113191:5244229-5244612 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT265540 WP_004150355.1 NZ_CP113191:5243987-5244229 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References