Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 28765..29290 Replicon plasmid pSYCC2_mcr-8_103k
Accession NZ_CP113187
Organism Klebsiella pneumoniae strain SYCC2

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag OT482_RS01640 Protein ID WP_013023785.1
Coordinates 28985..29290 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag OT482_RS01635 Protein ID WP_001568025.1
Coordinates 28765..28983 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OT482_RS01615 (OT482_01615) 24012..24980 + 969 WP_075211999.1 IS5 family transposase -
OT482_RS01620 (OT482_01620) 25452..26243 - 792 WP_221928726.1 ribbon-helix-helix domain-containing protein -
OT482_RS01625 (OT482_01625) 26426..27454 - 1029 WP_032445668.1 Abi family protein -
OT482_RS01630 (OT482_01630) 27615..28196 - 582 WP_072310991.1 hypothetical protein -
OT482_RS01635 (OT482_01635) 28765..28983 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
OT482_RS01640 (OT482_01640) 28985..29290 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
OT482_RS01645 (OT482_01645) 29459..29854 + 396 WP_017899885.1 hypothetical protein -
OT482_RS01650 (OT482_01650) 29881..30204 + 324 WP_004197641.1 hypothetical protein -
OT482_RS01655 (OT482_01655) 30201..31217 + 1017 WP_118842534.1 hypothetical protein -
OT482_RS01660 (OT482_01660) 31415..32200 + 786 WP_046664219.1 site-specific integrase -
OT482_RS01665 (OT482_01665) 32649..33404 + 756 WP_001568031.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-8 - 1..103507 103507
- flank IS/Tn mcr-8 - 17955..24980 7025


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T265520 WP_013023785.1 NZ_CP113187:28985-29290 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT265520 WP_001568025.1 NZ_CP113187:28765-28983 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References