Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4627000..4627810 Replicon chromosome
Accession NZ_CP113173
Organism Klebsiella pneumoniae strain TSYCC10-2

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag OT486_RS22805 Protein ID WP_023279404.1
Coordinates 4627000..4627533 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag OT486_RS22810 Protein ID WP_002887278.1
Coordinates 4627544..4627810 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OT486_RS22800 (4625831) 4625831..4626952 + 1122 WP_002887282.1 cupin domain-containing protein -
OT486_RS22805 (4627000) 4627000..4627533 - 534 WP_023279404.1 type II toxin-antitoxin system toxin KacT Toxin
OT486_RS22810 (4627544) 4627544..4627810 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
OT486_RS22815 (4627913) 4627913..4629346 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
OT486_RS22820 (4629336) 4629336..4630019 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
OT486_RS22825 (4630191) 4630191..4631576 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
OT486_RS22830 (4631594) 4631594..4631938 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T265491 WP_023279404.1 NZ_CP113173:c4627533-4627000 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT265491 WP_002887278.1 NZ_CP113173:c4627810-4627544 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References