Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3014032..3014686 Replicon chromosome
Accession NC_017328
Organism Shigella flexneri 2002017

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag SFXV_RS16475 Protein ID WP_000244767.1
Coordinates 3014032..3014439 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID D2A730
Locus tag SFXV_RS16480 Protein ID WP_000354052.1
Coordinates 3014420..3014686 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SFXV_RS16455 (3009989) 3009989..3011722 - 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -
SFXV_RS16460 (3011728) 3011728..3012438 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
SFXV_RS16465 (3012463) 3012463..3013359 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
SFXV_RS16470 (3013471) 3013471..3013992 + 522 WP_001055867.1 flavodoxin FldB -
SFXV_RS16475 (3014032) 3014032..3014439 - 408 WP_000244767.1 protein YgfX Toxin
SFXV_RS16480 (3014420) 3014420..3014686 - 267 WP_000354052.1 FAD assembly factor SdhE Antitoxin
SFXV_RS16485 (3014929) 3014929..3015909 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
SFXV_RS16490 (3016105) 3016105..3016764 - 660 WP_000250274.1 hemolysin III family protein -
SFXV_RS16495 (3016928) 3016928..3017238 - 311 Protein_3021 N(4)-acetylcytidine aminohydrolase -
SFXV_RS16500 (3017283) 3017283..3018716 + 1434 WP_005051722.1 6-phospho-beta-glucosidase BglA -
SFXV_RS16505 (3018773) 3018773..3019516 - 744 WP_000951957.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T26548 WP_000244767.1 NC_017328:c3014439-3014032 [Shigella flexneri 2002017]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T26548 NC_017328:c3014439-3014032 [Shigella flexneri 2002017]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10519.05 Da        Isoelectric Point: 5.1599

>AT26548 WP_000354052.1 NC_017328:c3014686-3014420 [Shigella flexneri 2002017]
MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT26548 NC_017328:c3014686-3014420 [Shigella flexneri 2002017]
ATGGACATTAACAACAAAGCCCGAATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGTCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB D2A730

References