Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 9422..9947 Replicon plasmid pTSNTC10-1_mcr-8_104
Accession NZ_CP113167
Organism Klebsiella pneumoniae strain TSNTC10-1

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag OT484_RS28045 Protein ID WP_013023785.1
Coordinates 9642..9947 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag OT484_RS28040 Protein ID WP_006788213.1
Coordinates 9422..9640 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OT484_RS28020 (OT484_28020) 4669..5637 + 969 WP_075211999.1 IS5 family transposase -
OT484_RS28025 (OT484_28025) 6109..6900 - 792 WP_221928726.1 ribbon-helix-helix domain-containing protein -
OT484_RS28030 (OT484_28030) 7083..8111 - 1029 WP_032445668.1 Abi family protein -
OT484_RS28035 (OT484_28035) 8272..8853 - 582 WP_072310991.1 hypothetical protein -
OT484_RS28040 (OT484_28040) 9422..9640 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
OT484_RS28045 (OT484_28045) 9642..9947 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
OT484_RS28050 (OT484_28050) 10116..10511 + 396 WP_017899885.1 hypothetical protein -
OT484_RS28055 (OT484_28055) 10538..10861 + 324 WP_004197641.1 hypothetical protein -
OT484_RS28060 (OT484_28060) 10858..11874 + 1017 WP_118842534.1 hypothetical protein -
OT484_RS28065 (OT484_28065) 12072..12857 + 786 WP_046664219.1 site-specific integrase -
OT484_RS28070 (OT484_28070) 13306..14061 + 756 WP_001568031.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-15 - 1..104215 104215
- flank IS/Tn - - 4669..5637 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T265463 WP_013023785.1 NZ_CP113167:9642-9947 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT265463 WP_006788213.1 NZ_CP113167:9422-9640 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References