Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 143346..144969 Replicon plasmid pLBA3-1
Accession NZ_CP113164
Organism Citrobacter braakii strain LBA3

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag MKJ05_RS24305 Protein ID WP_000088046.1
Coordinates 143644..144969 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag MKJ05_RS24300 Protein ID WP_024136327.1
Coordinates 143346..143639 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKJ05_RS24270 (MKJ05_24270) 139218..140459 + 1242 WP_001053338.1 VWA domain-containing protein -
MKJ05_RS24275 (MKJ05_24275) 140550..140681 + 132 WP_000374059.1 hypothetical protein -
MKJ05_RS24280 (MKJ05_24280) 141022..141303 - 282 WP_000077926.1 hypothetical protein -
MKJ05_RS24285 (MKJ05_24285) 141353..141544 - 192 WP_000398480.1 hypothetical protein -
MKJ05_RS24290 (MKJ05_24290) 141636..141977 - 342 WP_001151575.1 hypothetical protein -
MKJ05_RS24295 (MKJ05_24295) 142350..142742 + 393 WP_000341066.1 hypothetical protein -
MKJ05_RS24300 (MKJ05_24300) 143346..143639 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
MKJ05_RS24305 (MKJ05_24305) 143644..144969 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
MKJ05_RS24310 (MKJ05_24310) 145030..145236 + 207 WP_000134171.1 hypothetical protein -
MKJ05_RS24315 (MKJ05_24315) 145338..145748 + 411 WP_000985911.1 hypothetical protein -
MKJ05_RS24320 (MKJ05_24320) 145761..146576 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
MKJ05_RS24325 (MKJ05_24325) 146830..147255 + 426 WP_001043843.1 hypothetical protein -
MKJ05_RS24330 (MKJ05_24330) 147804..148112 + 309 WP_001224687.1 hypothetical protein -
MKJ05_RS24335 (MKJ05_24335) 148128..148985 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
MKJ05_RS24340 (MKJ05_24340) 149047..149250 + 204 WP_001194555.1 hypothetical protein -
MKJ05_RS24345 (MKJ05_24345) 149592..149948 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / msr(E) / mph(E) / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aph(4)-Ia / aac(3)-IVa - 1..237284 237284


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T265444 WP_000088046.1 NZ_CP113164:143644-144969 [Citrobacter braakii]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT265444 WP_024136327.1 NZ_CP113164:143346-143639 [Citrobacter braakii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References