265437

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1653269..1653859 Replicon chromosome
Accession NZ_CP113163
Organism Citrobacter braakii strain LBA3

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag MKJ05_RS07995 Protein ID WP_267589032.1
Coordinates 1653269..1653601 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag MKJ05_RS08000 Protein ID WP_267589033.1
Coordinates 1653602..1653859 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKJ05_RS07955 (MKJ05_07955) 1649416..1649873 + 458 Protein_1563 hypothetical protein -
MKJ05_RS07960 (MKJ05_07960) 1649853..1649999 + 147 WP_267589294.1 AlpA family phage regulatory protein -
MKJ05_RS07965 (MKJ05_07965) 1650021..1650202 + 182 Protein_1565 hypothetical protein -
MKJ05_RS07970 (MKJ05_07970) 1650187..1650500 + 314 Protein_1566 STY4526/YPO1902 family pathogenicity island replication protein -
MKJ05_RS07975 (MKJ05_07975) 1650490..1650702 + 213 WP_114682487.1 TraR/DksA family transcriptional regulator -
MKJ05_RS07980 (MKJ05_07980) 1650825..1651775 - 951 WP_016152661.1 HTH-type transcriptional regulator Cbl -
MKJ05_RS07985 (MKJ05_07985) 1651877..1652794 - 918 WP_267589031.1 nitrogen assimilation transcriptional regulator NAC -
MKJ05_RS07995 (MKJ05_07995) 1653269..1653601 - 333 WP_267589032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MKJ05_RS08000 (MKJ05_08000) 1653602..1653859 - 258 WP_267589033.1 antitoxin Antitoxin
MKJ05_RS08005 (MKJ05_08005) 1654471..1655556 + 1086 WP_267589034.1 hypothetical protein -
MKJ05_RS08010 (MKJ05_08010) 1655667..1658369 + 2703 WP_267589035.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1649703..1664763 15060


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11800.58 Da        Isoelectric Point: 10.2965

>T265437 WP_267589032.1 NZ_CP113163:c1653601-1653269 [Citrobacter braakii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPITSGGNFARTAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKRLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9289.78 Da        Isoelectric Point: 4.4012

>AT265437 WP_267589033.1 NZ_CP113163:c1653859-1653602 [Citrobacter braakii]
MYTTRLKKVGGSVMLAVPPAVLKTLALSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPQAEMSGEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References