Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 17972..18573 Replicon plasmid pBRV276
Accession NZ_CP113118
Organism Levilactobacillus brevis strain SMB091

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag ORR04_RS12250 Protein ID WP_001748110.1
Coordinates 17972..18316 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q684P2
Locus tag ORR04_RS12255 Protein ID WP_003643337.1
Coordinates 18310..18573 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORR04_RS12230 (ORR04_12230) 13881..15035 + 1155 WP_267668706.1 cation:proton antiporter -
ORR04_RS12235 (ORR04_12235) 15158..16726 + 1569 WP_267668708.1 ClC family H(+)/Cl(-) exchange transporter -
ORR04_RS12240 (ORR04_12240) 16832..16921 + 90 Protein_16 IS30 family transposase -
ORR04_RS12245 (ORR04_12245) 16963..17430 - 468 WP_233758948.1 DNA starvation/stationary phase protection protein -
ORR04_RS12250 (ORR04_12250) 17972..18316 - 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ORR04_RS12255 (ORR04_12255) 18310..18573 - 264 WP_003643337.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ORR04_RS12260 (ORR04_12260) 18512..18814 + 303 Protein_20 site-specific integrase -
ORR04_RS12270 (ORR04_12270) 19652..20104 + 453 WP_267668727.1 tyrosine-type recombinase/integrase -
ORR04_RS12275 (ORR04_12275) 20595..21434 + 840 Protein_23 IS30 family transposase -
ORR04_RS12280 (ORR04_12280) 21431..21634 - 204 WP_267668715.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..27635 27635


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T265431 WP_001748110.1 NZ_CP113118:c18316-17972 [Levilactobacillus brevis]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.51 Da        Isoelectric Point: 6.4708

>AT265431 WP_003643337.1 NZ_CP113118:c18573-18310 [Levilactobacillus brevis]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWKDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A6L4ZZT2

References