Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3866680..3867297 Replicon chromosome
Accession NZ_CP113085
Organism Pantoea agglomerans strain AB378

Toxin (Protein)


Gene name Hha Uniprot ID E0LTU7
Locus tag OSE17_RS17925 Protein ID WP_003850458.1
Coordinates 3867082..3867297 (+) Length 72 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID E0LTU8
Locus tag OSE17_RS17920 Protein ID WP_003850455.1
Coordinates 3866680..3867057 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OSE17_RS17890 (OSE17_17890) 3862987..3863241 + 255 WP_010255899.1 type B 50S ribosomal protein L31 -
OSE17_RS17895 (OSE17_17895) 3863253..3863393 + 141 WP_010255896.1 type B 50S ribosomal protein L36 -
OSE17_RS17900 (OSE17_17900) 3863439..3864317 - 879 WP_010671218.1 metal ABC transporter substrate-binding protein -
OSE17_RS17905 (OSE17_17905) 3864333..3865172 - 840 WP_010255890.1 metal ABC transporter permease -
OSE17_RS17910 (OSE17_17910) 3865169..3865837 - 669 WP_010255888.1 ABC transporter ATP-binding protein -
OSE17_RS17915 (OSE17_17915) 3866179..3866532 + 354 WP_010255885.1 hypothetical protein -
OSE17_RS17920 (OSE17_17920) 3866680..3867057 + 378 WP_003850455.1 Hha toxicity modulator TomB Antitoxin
OSE17_RS17925 (OSE17_17925) 3867082..3867297 + 216 WP_003850458.1 HHA domain-containing protein Toxin
OSE17_RS17935 (OSE17_17935) 3867704..3868015 + 312 WP_010255882.1 MGMT family protein -
OSE17_RS17940 (OSE17_17940) 3868055..3868612 - 558 WP_010255881.1 YbaY family lipoprotein -
OSE17_RS17945 (OSE17_17945) 3868803..3869666 + 864 WP_045140707.1 acyl-CoA thioesterase II -
OSE17_RS17950 (OSE17_17950) 3869718..3871004 - 1287 WP_010255877.1 ammonium transporter AmtB -
OSE17_RS17955 (OSE17_17955) 3871039..3871377 - 339 WP_003850469.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(12-68)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 72 a.a.        Molecular weight: 8510.90 Da        Isoelectric Point: 9.4825

>T265380 WP_003850458.1 NZ_CP113085:3867082-3867297 [Pantoea agglomerans]
MNKTLTKTDYLMRLRRCRSLDTLERVIEKNKYELPEDELAVFYSAADHRLAELTMNKLYDKVPGSVWKFVR

Download         Length: 216 bp

>T265380 NZ_CP113085:3867082-3867297 [Pantoea agglomerans]
ATGAACAAAACCCTGACTAAAACCGATTATTTGATGCGACTGCGGCGCTGCCGCTCTCTTGATACCCTGGAAAGGGTGAT
TGAAAAAAATAAGTATGAATTACCGGAAGATGAATTAGCGGTATTTTACTCAGCAGCTGACCATCGCCTGGCGGAACTGA
CGATGAACAAGCTCTACGATAAAGTCCCGGGTTCAGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 14636.25 Da        Isoelectric Point: 4.3976

>AT265380 WP_003850455.1 NZ_CP113085:3866680-3867057 [Pantoea agglomerans]
MDEYSPKRHDIAQLKYLCESLFDDSMATLTDSHHGWVNDPTSESNLQLNDLIEHIASFTMNYKIKHVEDEALISQIDEYL
DDTFMLFSNYGVNSPDLQRWQRSAKRLFNLFTEECAFLQQPSHSL

Download         Length: 378 bp

>AT265380 NZ_CP113085:3866680-3867057 [Pantoea agglomerans]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACCTTTGTGAGAGTCTGTTTGACGACAGCATGGC
AACATTAACCGACAGTCATCATGGCTGGGTTAATGATCCCACTTCTGAAAGCAATCTACAGCTTAACGATTTGATTGAGC
ATATCGCCTCTTTCACCATGAATTACAAAATTAAGCACGTTGAAGATGAAGCGCTGATTTCGCAAATCGATGAATATCTT
GATGATACTTTTATGCTATTCAGCAATTATGGTGTCAATAGTCCGGATCTTCAGCGGTGGCAACGTTCAGCAAAACGCTT
ATTTAATCTGTTCACTGAAGAGTGCGCTTTTCTCCAGCAACCGAGCCATTCATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I5AGC3

Antitoxin

Source ID Structure
AlphaFold DB A0A1I5AG55

References