Detailed information of TA system TA026530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 1336199..1336794
• Replicon: plasmid pSmeSM11c
• Accession: NC_017327
• Organism: Sinorhizobium meliloti SM11

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F7XBJ0
• Locus tag: SM11_RS25425
• Protein ID: WP_014531932.1
• Coordinates: 1336199..1336489 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q930F1
• Locus tag: SM11_RS25430
• Protein ID: WP_010967242.1
• Coordinates: 1336486..1336794 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM11_RS25405	1331774..1332673	+	900	WP_010967198.1	LysR family transcriptional regulator	-
SM11_RS36610	1333115..1334115	+	1001	Protein_1287	IS30 family transposase	-
SM11_RS36615	1334207..1334464	-	258	WP_080567622.1	hypothetical protein	-
SM11_RS25420	1334738..1335937	+	1200	WP_014531931.1	NAD-dependent formate dehydrogenase	-
SM11_RS25425	1336199..1336489	-	291	WP_014531932.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
SM11_RS25430	1336486..1336794	-	309	WP_010967242.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SM11_RS25435	1336994..1337476	-	483	WP_017275386.1	hypothetical protein	-
SM11_RS39045	1337594..1338478	+	885	WP_100220003.1	hypothetical protein	-
SM11_RS25445	1338605..1339681	-	1077	WP_014531934.1	ISAs1-like element ISRm21 family transposase	-
SM11_RS25450	1339803..1340888	-	1086	WP_041862569.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1633319	1633319
	-	inside	IScluster/Tn	-	-	1333741..1339681	5940

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11195.84 Da
• Isoelectric Point: 6.4734

>T26530 WP_014531932.1 NC_017327:c1336489-1336199 [Sinorhizobium meliloti SM11]
MRLVWARYALDDRDTIFSYIERENPRAAVHVDEEIVSAVRRLLDFPESGRPGRIAATRELVIPRTYIAAYMVMEDRIRIL
RVLHGAQKWPSELDDG

Nucleotide

• Length: 291 bp

>T26530 NC_017327:c1336489-1336199 [Sinorhizobium meliloti SM11]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGCAACTCGAGAGCTTGTTATCCCTCGGACCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATTCTG
CGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11210.58 Da
• Isoelectric Point: 5.6604

>AT26530 WP_010967242.1 NC_017327:c1336794-1336486 [Sinorhizobium meliloti SM11]
MASNALVQTRIDAEVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELFSHSEAHDAWFRAKVLRALEDTRPDVDDA
DADAHFRERRAAALRKAAAGDR

Nucleotide

• Length: 309 bp

>AT26530 NC_017327:c1336794-1336486 [Sinorhizobium meliloti SM11]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA

Structures

Toxin

• AlphaFold DB: F7XBJ0

Antitoxin

• AlphaFold DB: Q930F1