Detailed information of TA system TA026529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 906178..906863
• Replicon: plasmid pSmeSM11c
• Accession: NC_017327
• Organism: Sinorhizobium meliloti SM11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SM11_RS23445
• Protein ID: WP_014531572.1
• Coordinates: 906444..906863 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XET8
• Locus tag: SM11_RS23440
• Protein ID: WP_013845417.1
• Coordinates: 906178..906447 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM11_RS36155	901227..901586	-	360	Protein_879	FAD:protein FMN transferase	-
SM11_RS23415	901696..902565	-	870	WP_014528623.1	aldo/keto reductase family oxidoreductase	-
SM11_RS23420	902682..903605	+	924	WP_013845420.1	LysR family transcriptional regulator	-
SM11_RS23430	904585..905460	+	876	WP_014531570.1	LysR family transcriptional regulator	-
SM11_RS36165	905548..906040	-	493	Protein_883	LysR family transcriptional regulator	-
SM11_RS23440	906178..906447	+	270	WP_013845417.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SM11_RS23445	906444..906863	+	420	WP_014531572.1	type II toxin-antitoxin system VapC family toxin	Toxin
SM11_RS23450	907017..907430	-	414	WP_014531574.1	hypothetical protein	-
SM11_RS23455	907700..908011	+	312	WP_017266244.1	type II toxin-antitoxin system HigB family toxin	-
SM11_RS23460	908029..908409	+	381	WP_003526626.1	transcriptional regulator	-
SM11_RS23465	908669..909670	+	1002	WP_014531093.1	IS30 family transposase	-
SM11_RS23470	909796..911169	+	1374	WP_014531577.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1633319	1633319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14778.09 Da
• Isoelectric Point: 4.8721

>T26529 WP_014531572.1 NC_017327:906444-906863 [Sinorhizobium meliloti SM11]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSC

Nucleotide

• Length: 420 bp

>T26529 NC_017327:906444-906863 [Sinorhizobium meliloti SM11]
GTGAGCAGGCTCTACATGCTGGACACCAATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CATCGAAACTACTGGCGCAGATCGAGACCATTCTTGGAAGTATGCAAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCCGCTGGTAAGCCCATCGGCCCGAATGACCTGTTCATCGCCGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACGGCCAACAGTAGCGAGTTCACACGCGTTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTCCTTCGTGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10203.67 Da
• Isoelectric Point: 4.4798

>AT26529 WP_013845417.1 NC_017327:906178-906447 [Sinorhizobium meliloti SM11]
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT26529 NC_017327:906178-906447 [Sinorhizobium meliloti SM11]
ATGCCCGAACTCCGACACAACGAACCAACACCGCCAAGGGAAGCTAAGCTCTTTCGAAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCCGGCGATCGGGTGATGATCCATCGGGACGGAGATCGGCTGATAATAGAGCCGGTGC
TCCGCAAGAACTTGCTGGAGGTGCTGGCTGGTTTGGAGCCGCTCGGGTCTGAAGATGAGTTCCCCGACGTTGACGAAACC
CTGCTGCCGGTGAAGGAAATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A844A3Y5