Detailed information of TA system TA026528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 578450..578949
• Replicon: plasmid pSmeSM11c
• Accession: NC_017327
• Organism: Sinorhizobium meliloti SM11

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q92YA6
• Locus tag: SM11_RS21900
• Protein ID: WP_010967939.1
• Coordinates: 578776..578949 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: SM11_RS39570
• Protein ID: WP_017272263.1
• Coordinates: 578450..578779 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM11_RS21875	574127..575020	-	894	WP_010967943.1	LysR family transcriptional regulator	-
SM11_RS21880	575112..575555	+	444	WP_010967942.1	PACE efflux transporter	-
SM11_RS21885	575672..576010	+	339	WP_013845641.1	SDR family oxidoreductase	-
SM11_RS36010	575994..576335	-	342	Protein_577	hypothetical protein	-
SM11_RS21890	576719..578089	-	1371	WP_017265792.1	alpha/beta hydrolase	-
SM11_RS36015	578330..578515	-	186	WP_013845639.1	hypothetical protein	-
SM11_RS39570	578450..578779	+	330	WP_017272263.1	hypothetical protein	Antitoxin
SM11_RS21900	578776..578949	+	174	WP_010967939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SM11_RS21905	579047..579352	-	306	WP_013845638.1	hypothetical protein	-
SM11_RS21910	579438..579677	-	240	WP_017272264.1	hypothetical protein	-
SM11_RS21915	579670..580029	-	360	WP_013845635.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
SM11_RS21920	580624..580875	+	252	WP_003534243.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
SM11_RS21925	580903..581388	+	486	WP_014531392.1	YidB family protein	-
SM11_RS21930	581758..583074	-	1317	WP_014531394.1	aspartate aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1633319	1633319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6497.32 Da
• Isoelectric Point: 5.7733

>T26528 WP_010967939.1 NC_017327:578776-578949 [Sinorhizobium meliloti SM11]
MKLVWARYALDDRDAIFSYIERENPRAAVHVDEEVVSAGRPLDFPESRRPGRIAGTP

Nucleotide

• Length: 174 bp

>T26528 NC_017327:578776-578949 [Sinorhizobium meliloti SM11]
ATGAAGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACGCCATCTTCAGCTACATCGAACGAGAGAATCCGAGGGC
GGCGGTCCACGTTGACGAGGAGGTCGTCAGCGCTGGTCGTCCGCTGGACTTTCCCGAAAGCCGCCGCCCTGGCCGGATTG
CCGGAACTCCATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12025.64 Da
• Isoelectric Point: 11.1401

>AT26528 WP_017272263.1 NC_017327:578450-578779 [Sinorhizobium meliloti SM11]
VSERLLLIALLALGPFRASQRHTGRPALLRRGPQSAAGAVEEHFIQLRAPLWLITLGGFSHREAHDAWFRAKALRALEDT
RPDADDADADAHFQERRAAALRKAAAGDR

Nucleotide

• Length: 330 bp

>AT26528 NC_017327:578450-578779 [Sinorhizobium meliloti SM11]
GTGAGCGAGCGCCTTCTGCTCATCGCCCTCCTGGCCCTCGGTCCGTTCCGAGCCAGCCAGCGGCATACCGGTCGTCCTGC
TCTGCTACGGCGTGGCCCCCAATCGGCCGCCGGCGCGGTCGAGGAGCACTTCATCCAACTCCGCGCTCCGCTGTGGCTGA
TCACGCTCGGCGGTTTCAGCCACAGAGAGGCTCACGACGCTTGGTTCCGCGCCAAAGCTTTGCGGGCACTCGAGGACACT
CGACCCGATGCGGACGACGCCGACGCCGATGCGCACTTTCAAGAGCGCCGCGCCGCAGCTTTGCGCAAGGCTGCGGCGGG
TGATCGATGA

Structures

Toxin

• AlphaFold DB: Q92YA6