Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 296463..297043 Replicon plasmid pSmeSM11c
Accession NC_017327
Organism Sinorhizobium meliloti SM11

Toxin (Protein)


Gene name vapC Uniprot ID Q92XP1
Locus tag SM11_RS20475 Protein ID WP_010968151.1
Coordinates 296463..296846 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID F7XCL0
Locus tag SM11_RS20480 Protein ID WP_013851145.1
Coordinates 296843..297043 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SM11_RS38730 291558..291839 + 282 Protein_290 AI-2E family transporter -
SM11_RS20445 291844..292788 + 945 Protein_291 AI-2E family transporter -
SM11_RS20450 293164..293574 - 411 WP_014531156.1 hypothetical protein -
SM11_RS20460 294136..294411 + 276 WP_014531159.1 hypothetical protein -
SM11_RS20465 295457..295663 - 207 WP_013845026.1 CsbD family protein -
SM11_RS20470 295857..296117 + 261 WP_013845025.1 hypothetical protein -
SM11_RS20475 296463..296846 - 384 WP_010968151.1 type II toxin-antitoxin system VapC family toxin Toxin
SM11_RS20480 296843..297043 - 201 WP_013851145.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
SM11_RS35775 297334..297735 + 402 WP_014531161.1 GFA family protein -
SM11_RS20485 297812..298648 - 837 WP_014531162.1 helix-turn-helix transcriptional regulator -
SM11_RS35780 298744..299067 + 324 WP_017266777.1 DUF1127 domain-containing protein -
SM11_RS20495 299106..299834 + 729 WP_014531163.1 class I SAM-dependent methyltransferase -
SM11_RS20500 299928..300074 + 147 WP_017266778.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB / htpB 1..1633319 1633319


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14292.54 Da        Isoelectric Point: 6.9172

>T26527 WP_010968151.1 NC_017327:c296846-296463 [Sinorhizobium meliloti SM11]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Download         Length: 384 bp

>T26527 NC_017327:c296846-296463 [Sinorhizobium meliloti SM11]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7096.22 Da        Isoelectric Point: 11.1107

>AT26527 WP_013851145.1 NC_017327:c297043-296843 [Sinorhizobium meliloti SM11]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT26527 NC_017327:c297043-296843 [Sinorhizobium meliloti SM11]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGTCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92XP1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6MID2

References