Detailed information of TA system TA026526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 1619429..1619963
• Replicon: plasmid pSmeSM11d
• Accession: NC_017326
• Organism: Sinorhizobium meliloti SM11

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q92X99
• Locus tag: SM11_RS34275
• Protein ID: WP_010974986.1
• Coordinates: 1619429..1619725 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: SM11_RS34280
• Protein ID: WP_003527850.1
• Coordinates: 1619715..1619963 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM11_RS34250	1614871..1616274	-	1404	WP_041862735.1	sodium:alanine symporter family protein	-
SM11_RS34255	1616808..1617017	-	210	WP_003527838.1	dodecin family protein	-
SM11_RS34260	1617307..1617753	+	447	WP_014527651.1	DNA-binding protein	-
SM11_RS34265	1617757..1617942	-	186	WP_010974989.1	hypothetical protein	-
SM11_RS34270	1618109..1618507	-	399	WP_010974988.1	hypothetical protein	-
SM11_RS37520	1619088..1619348	+	261	Protein_1479	helix-turn-helix domain containing protein	-
SM11_RS34275	1619429..1619725	-	297	WP_010974986.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SM11_RS34280	1619715..1619963	-	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
SM11_RS39260	1620071..1620226	-	156	WP_010974985.1	hypothetical protein	-
SM11_RS34290	1620582..1620707	+	126	Protein_1483	IS481 family transposase	-
SM11_RS34295	1620791..1621585	-	795	WP_013850206.1	methyltransferase domain-containing protein	-
SM11_RS34300	1621589..1622389	-	801	WP_014530872.1	ABC transporter ATP-binding protein	-
SM11_RS34305	1622386..1623429	-	1044	WP_010974980.1	iron ABC transporter permease	-
SM11_RS34310	1623426..1624511	-	1086	WP_014530873.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..1632395	1632395
	-	flank	IS/Tn	-	-	1620516..1620707	191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-91)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11473.15 Da
• Isoelectric Point: 10.6593

>T26526 WP_010974986.1 NC_017326:c1619725-1619429 [Sinorhizobium meliloti SM11]
MATERPFRLAPTAKADLRKIWRYTARRWSLEQAETYQDQLYTAFEGLAVGTKKGRNVDVRPGYLKYPAGAHIVYFRDRGD
RIDIIRILHGRVDAQRHL

Nucleotide

• Length: 297 bp

>T26526 NC_017326:c1619725-1619429 [Sinorhizobium meliloti SM11]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTACCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACACGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGTAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGGCCGGGTTATCTCAAATACCCGGCAGGAGCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGTGTGGACGCCCAACGGCATTTGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT26526 WP_003527850.1 NC_017326:c1619963-1619715 [Sinorhizobium meliloti SM11]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT26526 NC_017326:c1619963-1619715 [Sinorhizobium meliloti SM11]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CCTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Toxin

• AlphaFold DB: Q92X99

Antitoxin

• AlphaFold DB: H0FXK0