Detailed information of TA system TA026524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3468520..3469181
• Replicon: chromosome
• Accession: NC_017325
• Organism: Sinorhizobium meliloti SM11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92KY5
• Locus tag: SM11_RS16965
• Protein ID: WP_010970565.1
• Coordinates: 3468520..3468924 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92KY4
• Locus tag: SM11_RS16970
• Protein ID: WP_010970566.1
• Coordinates: 3468921..3469181 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM11_RS16950	3464061..3465173	+	1113	WP_014529973.1	3-isopropylmalate dehydrogenase	-
SM11_RS16955	3465302..3466009	+	708	WP_010970563.1	phosphatase PAP2 family protein	-
SM11_RS16960	3466110..3467999	-	1890	WP_014527006.1	MHS family MFS transporter	-
SM11_RS16965	3468520..3468924	-	405	WP_010970565.1	type II toxin-antitoxin system VapC family toxin	Toxin
SM11_RS16970	3468921..3469181	-	261	WP_010970566.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SM11_RS35405	3469310..3469480	+	171	WP_010970567.1	hypothetical protein	-
SM11_RS16975	3469594..3470628	+	1035	WP_003531790.1	aspartate-semialdehyde dehydrogenase	-
SM11_RS16980	3470801..3471622	+	822	WP_013844939.1	lytic transglycosylase family protein	-
SM11_RS16985	3471969..3472652	-	684	WP_010970569.1	carbonic anhydrase	-
SM11_RS16990	3472826..3473707	-	882	WP_010970570.1	pyridoxal kinase PdxY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (8-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14507.71 Da
• Isoelectric Point: 6.7257

>T26524 WP_010970565.1 NC_017325:c3468924-3468520 [Sinorhizobium meliloti SM11]
VISHILDTNAVIALIGRKSDALVTRVLHSPQGIIGLPSVVAYELYFGAQKSAKAQHNLETLRLLMADFPILDFDRNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLRVEDWSL

Nucleotide

• Length: 405 bp

>T26524 NC_017325:c3468924-3468520 [Sinorhizobium meliloti SM11]
GTGATCTCCCATATTCTCGACACCAACGCCGTCATCGCTTTGATCGGGCGCAAATCGGATGCATTGGTCACCCGTGTACT
CCATAGCCCTCAGGGCATTATCGGGCTCCCGTCGGTCGTCGCGTATGAACTCTATTTTGGAGCGCAGAAAAGCGCGAAGG
CCCAACACAATCTCGAAACCCTGCGTTTGTTGATGGCGGATTTTCCGATCCTCGACTTCGACCGGAACGATGCCTTTGTC
GCAGGCGAGATCCGGGCCGCCCTTGCCGCGAAGGGTACGCCCATCGGACCTTATGACGTGCTGATTGCGGGACAGGCCAA
AGCCCGTGGATTGACGCTCGTCACCAACAATGTCGGCGAGTTCAATAGGGTTGAGAACCTGCGCGTGGAGGATTGGTCGC
TCTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9903.16 Da
• Isoelectric Point: 5.1253

>AT26524 WP_010970566.1 NC_017325:c3469181-3468921 [Sinorhizobium meliloti SM11]
MPHLARVFQSGNSQAVRLPKEFRFNVDRVEITQEGDALILRPHVEQGEQWSSLKAALARGMSEDFMMCGREQPEQQDRSE
LDAVFR

Nucleotide

• Length: 261 bp

>AT26524 NC_017325:c3469181-3468921 [Sinorhizobium meliloti SM11]
ATGCCCCATCTTGCACGGGTGTTCCAGTCCGGAAATTCGCAGGCCGTCCGCCTGCCTAAGGAATTCCGCTTCAACGTCGA
TCGCGTCGAAATCACGCAGGAAGGCGACGCGCTCATTCTCCGCCCGCACGTCGAGCAAGGCGAGCAGTGGTCATCGCTGA
AGGCGGCACTTGCCCGTGGCATGAGTGAAGACTTCATGATGTGCGGGCGCGAGCAGCCGGAACAGCAGGATCGGTCGGAA
CTCGACGCGGTATTTCGGTGA

Structures

Toxin

• AlphaFold DB: Q92KY5

Antitoxin

• AlphaFold DB: Q92KY4