Detailed information of TA system TA026523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2820028..2820659
• Replicon: chromosome
• Accession: NC_017325
• Organism: Sinorhizobium meliloti SM11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SM11_RS14065
• Protein ID: WP_013844828.1
• Coordinates: 2820028..2820426 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7X7L9
• Locus tag: SM11_RS14070
• Protein ID: WP_014529879.1
• Coordinates: 2820426..2820659 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM11_RS14030	2815110..2815412	-	303	WP_010970122.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SM11_RS14035	2815409..2815684	-	276	WP_010970123.1	type II toxin-antitoxin system ParD family antitoxin	-
SM11_RS35285	2815970..2816119	+	150	WP_010970124.1	hypothetical protein	-
SM11_RS14045	2816116..2817051	+	936	WP_010970125.1	site-specific tyrosine recombinase XerD	-
SM11_RS14050	2817115..2818068	+	954	WP_003527479.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
SM11_RS14055	2818313..2819755	+	1443	WP_014529878.1	murein L,D-transpeptidase	-
SM11_RS14060	2819755..2819994	+	240	WP_010970127.1	sulfurtransferase TusA family protein	-
SM11_RS14065	2820028..2820426	-	399	WP_013844828.1	type II toxin-antitoxin system toxin VapC	Toxin
SM11_RS14070	2820426..2820659	-	234	WP_014529879.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SM11_RS14075	2820768..2821913	-	1146	WP_010970130.1	GTP-binding protein	-
SM11_RS14080	2821927..2823009	-	1083	WP_017267062.1	D-alanyl-D-alanine carboxypeptidase	-
SM11_RS14085	2823261..2824424	+	1164	WP_010970132.1	amidohydrolase	-
SM11_RS14090	2824447..2824911	+	465	WP_010970133.1	Lrp/AsnC ligand binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14829.13 Da
• Isoelectric Point: 6.9581

>T26523 WP_013844828.1 NC_017325:c2820426-2820028 [Sinorhizobium meliloti SM11]
MLTYMLDTNICIYVMKTYPPAVREKFNGLAEQLCISSITLGELHYGAEKSARRVENLTAIEHFVARLEVLPFADKAAAHY
GQVRAELERTGTPCGPHDMQIGAHARSEGLIVVTNNIREFVRMPGVRVENWL

Nucleotide

• Length: 399 bp

>T26523 NC_017325:c2820426-2820028 [Sinorhizobium meliloti SM11]
ATGCTCACCTATATGCTCGATACCAATATCTGCATCTATGTGATGAAGACCTATCCGCCGGCCGTGCGCGAAAAGTTCAA
CGGACTGGCGGAGCAGCTTTGCATATCGAGCATCACGCTGGGAGAATTACACTATGGCGCCGAAAAATCGGCCCGGCGCG
TTGAGAACCTTACGGCTATCGAACATTTCGTGGCACGGCTGGAGGTGCTGCCGTTCGCAGACAAGGCAGCGGCCCACTAC
GGGCAGGTTCGGGCTGAACTGGAGCGGACGGGAACGCCATGCGGCCCTCATGACATGCAGATCGGCGCGCATGCGCGCAG
CGAAGGGCTGATTGTCGTGACCAACAATATACGCGAGTTCGTCCGCATGCCGGGTGTAAGGGTCGAGAACTGGCTGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8674.86 Da
• Isoelectric Point: 7.0131

>AT26523 WP_014529879.1 NC_017325:c2820659-2820426 [Sinorhizobium meliloti SM11]
MASSTVFISNRSQAVRLPKAVAFPEGVHEVDILKIGRSRVIVPQGKRWDDLFLSGPRVSEDFMSERDQPVAETRESF

Nucleotide

• Length: 234 bp

>AT26523 NC_017325:c2820659-2820426 [Sinorhizobium meliloti SM11]
TTGGCCAGTTCGACCGTATTCATCAGTAACCGTAGCCAGGCGGTGCGGCTGCCAAAGGCCGTCGCCTTTCCCGAGGGCGT
GCATGAGGTCGATATCCTCAAGATCGGCCGCAGCCGCGTGATCGTCCCCCAGGGCAAAAGATGGGACGATCTGTTCCTCA
GCGGGCCGCGCGTCAGCGAGGATTTCATGAGCGAGCGTGATCAGCCGGTGGCGGAAACACGCGAATCCTTCTGA

Structures

Antitoxin

• AlphaFold DB: F7X7L9