Detailed information of TA system TA026521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 2540233..2540906
• Replicon: chromosome
• Accession: NC_017325
• Organism: Sinorhizobium meliloti SM11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SM11_RS12675
• Protein ID: WP_010969926.1
• Coordinates: 2540502..2540906 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H0GA10
• Locus tag: SM11_RS12670
• Protein ID: WP_003536345.1
• Coordinates: 2540233..2540505 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM11_RS12650	2536224..2537864	-	1641	WP_003536336.1	ABC transporter substrate-binding protein	-
SM11_RS12655	2537912..2538967	-	1056	WP_014529838.1	dipeptidase	-
SM11_RS12660	2539147..2539488	-	342	WP_003536339.1	EamA family transporter	-
SM11_RS12665	2539536..2540096	-	561	WP_010969925.1	TetR/AcrR family transcriptional regulator	-
SM11_RS12670	2540233..2540505	+	273	WP_003536345.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SM11_RS12675	2540502..2540906	+	405	WP_010969926.1	type II toxin-antitoxin system VapC family toxin	Toxin
SM11_RS12680	2541034..2541426	+	393	WP_003536349.1	globin	-
SM11_RS12685	2541432..2541806	+	375	WP_010969927.1	DUF423 domain-containing protein	-
SM11_RS12690	2541929..2542183	+	255	WP_014529839.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
SM11_RS12695	2542299..2543828	+	1530	WP_012477342.1	IS21 family transposase	-
SM11_RS12700	2543841..2544578	+	738	WP_014529841.1	IS21-like element helper ATPase IstB	-
SM11_RS12705	2544861..2545175	-	315	WP_003536354.1	antibiotic biosynthesis monooxygenase	-
SM11_RS12710	2545188..2545454	-	267	WP_010969929.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14747.98 Da
• Isoelectric Point: 5.1974

>T26521 WP_010969926.1 NC_017325:2540502-2540906 [Sinorhizobium meliloti SM11]
MNGYLLDTNIISDVIHNPFGPAAQRIERIGPKEIYTSIVVASELRYGCAKKGSAKLLAKVESLLEIVPVLPLDIPADTRY
GSIRAELESLGQTIGSNDLLIAAHAYALDLTLVTDNIREFSRVRGLSLENWLER

Nucleotide

• Length: 405 bp

>T26521 NC_017325:2540502-2540906 [Sinorhizobium meliloti SM11]
TTGAACGGCTATCTTCTCGATACGAACATCATCAGCGACGTCATTCACAATCCGTTCGGACCGGCTGCGCAACGCATCGA
ACGGATCGGCCCGAAGGAGATTTACACCAGCATCGTCGTCGCGTCCGAGTTGCGGTATGGTTGCGCAAAAAAGGGATCGG
CGAAGTTGCTCGCCAAGGTCGAAAGCTTGCTGGAAATCGTCCCGGTGCTGCCATTGGATATTCCGGCCGACACCCGATAT
GGCAGCATTCGTGCGGAATTGGAGTCTCTGGGGCAGACCATCGGCTCCAACGATCTGTTGATTGCCGCGCATGCATATGC
GCTGGACCTGACGCTGGTGACGGACAATATCCGTGAGTTCAGCCGCGTTCGTGGCCTTAGTCTGGAAAACTGGCTTGAGC
GATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10334.02 Da
• Isoelectric Point: 6.3950

>AT26521 WP_003536345.1 NC_017325:2540233-2540505 [Sinorhizobium meliloti SM11]
MPVPLPSSRPKEVKLFRNNRSQAVRIPAEFELPGDRVLIRREGTRLIIEPIARPADIVELLAEWKKEAPLGPEDRFPDVE
DIPARPEKIF

Nucleotide

• Length: 273 bp

>AT26521 NC_017325:2540233-2540505 [Sinorhizobium meliloti SM11]
ATGCCCGTCCCGTTGCCGTCATCGCGACCTAAAGAAGTAAAGCTGTTCCGCAATAACCGCAGCCAGGCAGTCCGTATTCC
TGCGGAGTTCGAGTTGCCTGGAGACCGTGTGTTGATCCGTCGCGAAGGAACGAGACTCATTATCGAACCGATAGCCAGGC
CAGCCGATATCGTGGAGCTTCTCGCGGAGTGGAAGAAGGAAGCTCCCCTCGGACCTGAAGACCGGTTCCCGGATGTTGAG
GATATACCCGCCCGGCCGGAGAAAATCTTTTGA

Structures

Antitoxin

• AlphaFold DB: H0GA10