Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-RHH
Location 2269853..2270427 Replicon chromosome
Accession NC_017325
Organism Sinorhizobium meliloti SM11

Toxin (Protein)


Gene name vapC Uniprot ID Q92QN4
Locus tag SM11_RS11250 Protein ID WP_010969154.1
Coordinates 2270062..2270427 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q92QN3
Locus tag SM11_RS11245 Protein ID WP_010969155.1
Coordinates 2269853..2270068 (+) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SM11_RS11230 2265778..2266464 - 687 WP_010969157.1 ABC transporter ATP-binding protein -
SM11_RS11235 2266482..2267792 - 1311 WP_014529722.1 lipoprotein-releasing ABC transporter permease subunit -
SM11_RS11240 2267813..2269141 - 1329 WP_003531873.1 proline--tRNA ligase -
SM11_RS11245 2269853..2270068 + 216 WP_010969155.1 ribbon-helix-helix protein, CopG family Antitoxin
SM11_RS11250 2270062..2270427 + 366 WP_010969154.1 type II toxin-antitoxin system VapC family toxin Toxin
SM11_RS11255 2270514..2270783 - 270 WP_014526683.1 DUF1467 family protein -
SM11_RS11260 2270923..2271327 - 405 WP_010969152.1 methylmalonyl-CoA epimerase -
SM11_RS11265 2271386..2273053 - 1668 WP_010969151.1 ribonuclease J -
SM11_RS11270 2273081..2273848 - 768 WP_014529723.1 biotin--[acetyl-CoA-carboxylase] ligase -
SM11_RS11275 2273845..2275287 - 1443 WP_010969150.1 NADH-quinone oxidoreductase subunit NuoN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-40)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13592.57 Da        Isoelectric Point: 5.1787

>T26520 WP_010969154.1 NC_017325:2270062-2270427 [Sinorhizobium meliloti SM11]
MVGALFDTNILIDHLNAVPQAHKELDRFENRAISIITWMEVMVGADAELVEPTRRFLDGFETIALNDEIANRAVTLRRAH
RIKLPDAVIWATAQTAGRLLVTRNTKDFPADDPGIREPYAV

Download         Length: 366 bp

>T26520 NC_017325:2270062-2270427 [Sinorhizobium meliloti SM11]
ATGGTAGGCGCACTGTTCGATACCAATATCCTTATCGATCATCTGAATGCGGTGCCTCAGGCGCACAAGGAACTTGACCG
TTTCGAAAACCGGGCCATCAGCATCATCACCTGGATGGAGGTGATGGTTGGAGCGGACGCCGAACTGGTTGAGCCGACCC
GGCGCTTCCTCGACGGTTTCGAAACGATCGCCTTGAACGATGAGATCGCCAACCGTGCGGTGACATTGCGCAGGGCGCAC
CGCATCAAGCTACCCGACGCGGTGATCTGGGCAACGGCGCAGACTGCCGGTCGTCTGCTCGTGACGCGCAATACCAAGGA
CTTTCCAGCCGACGACCCGGGGATACGCGAACCCTACGCCGTGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 8381.51 Da        Isoelectric Point: 8.6052

>AT26520 WP_010969155.1 NC_017325:2269853-2270068 [Sinorhizobium meliloti SM11]
MRALIDMNDTQVEALDTLAKRVRRSRAALIREAIDDYLNRHHREQIEDGFGLWGKRKVDGLAYQEKVRGEW

Download         Length: 216 bp

>AT26520 NC_017325:2269853-2270068 [Sinorhizobium meliloti SM11]
ATGCGAGCCTTGATCGATATGAACGATACCCAGGTCGAAGCCCTAGATACCCTCGCCAAGCGGGTGCGCCGGTCCCGTGC
GGCTCTGATCCGCGAAGCGATCGACGACTATCTCAACCGTCATCATCGCGAGCAGATCGAAGATGGCTTCGGTTTGTGGG
GCAAGCGCAAAGTTGACGGTCTGGCTTATCAGGAGAAGGTGCGCGGCGAATGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92QN4

Antitoxin

Source ID Structure
AlphaFold DB Q92QN3

References