Detailed information of TA system TA026515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 267523..268100
• Replicon: chromosome
• Accession: NC_017325
• Organism: Sinorhizobium meliloti SM11

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: SM11_RS34535
• Protein ID: WP_014528972.1
• Coordinates: 267523..267786 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q92KN5
• Locus tag: SM11_RS01240
• Protein ID: WP_010968708.1
• Coordinates: 267783..268100 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM11_RS01220	263449..264978	+	1530	WP_014531024.1	IS21 family transposase	-
SM11_RS01225	264991..265728	+	738	WP_012477343.1	IS21-like element helper ATPase IstB	-
SM11_RS01230	265775..266872	-	1098	WP_014528970.1	AAA family ATPase	-
SM11_RS01235	266853..267095	-	243	WP_041862269.1	hypothetical protein	-
SM11_RS34535	267523..267786	+	264	WP_014528972.1	BrnT family toxin	Toxin
SM11_RS01240	267783..268100	+	318	WP_010968708.1	BrnA antitoxin family protein	Antitoxin
SM11_RS01245	268303..268767	-	465	WP_013844019.1	type II toxin-antitoxin system HicB family antitoxin	-
SM11_RS34540	268764..268952	-	189	WP_017266533.1	hypothetical protein	-
SM11_RS01250	269271..270371	+	1101	WP_014528974.1	hypothetical protein	-
SM11_RS01255	270358..271440	+	1083	WP_014528975.1	nucleotidyltransferase family protein	-
SM11_RS01260	271644..271955	+	312	WP_014528976.1	hypothetical protein	-
SM11_RS01270	272234..272622	-	389	Protein_250	DUF930 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	245331..268952	23621

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-103)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.16 Da
• Isoelectric Point: 10.3413

>T26515 WP_014528972.1 NC_017325:267523-267786 [Sinorhizobium meliloti SM11]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASAIIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Nucleotide

• Length: 264 bp

>T26515 NC_017325:267523-267786 [Sinorhizobium meliloti SM11]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCATCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12197.83 Da
• Isoelectric Point: 10.0883

>AT26515 WP_010968708.1 NC_017325:267783-268100 [Sinorhizobium meliloti SM11]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLPPEFFKAMEEHRRSRGRPSLEHPKKQVTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Nucleotide

• Length: 318 bp

>AT26515 NC_017325:267783-268100 [Sinorhizobium meliloti SM11]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCAAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG

Structures

Antitoxin

• AlphaFold DB: Q92KN5