Detailed information of TA system TA026514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1583190..1583689
• Replicon: plasmid pSINMEB01
• Accession: NC_017324
• Organism: Sinorhizobium meliloti BL225C

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q92YA6
• Locus tag: SINMEB_RS24995
• Protein ID: WP_010967939.1
• Coordinates: 1583190..1583363 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: SINMEB_RS37685
• Protein ID: WP_017268224.1
• Coordinates: 1583360..1583689 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINMEB_RS24970	1579058..1580374	+	1317	WP_013845634.1	aspartate aminotransferase family protein	-
SINMEB_RS24975	1580750..1581235	-	486	WP_014528881.1	YidB family protein	-
SINMEB_RS24980	1581263..1581514	-	252	WP_003534243.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
SINMEB_RS24985	1582109..1582468	+	360	WP_013845635.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
SINMEB_RS24990	1582787..1583092	+	306	WP_013845638.1	hypothetical protein	-
SINMEB_RS24995	1583190..1583363	-	174	WP_010967939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SINMEB_RS37685	1583360..1583689	-	330	WP_017268224.1	hypothetical protein	Antitoxin
SINMEB_RS35090	1583624..1583809	+	186	WP_013845639.1	hypothetical protein	-
SINMEB_RS25005	1584050..1585420	+	1371	WP_013845640.1	alpha/beta hydrolase	-
SINMEB_RS35095	1585804..1586146	+	343	Protein_1536	hypothetical protein	-
SINMEB_RS25010	1586130..1586468	-	339	WP_013845641.1	SDR family oxidoreductase	-
SINMEB_RS25015	1586585..1587028	-	444	WP_010967942.1	PACE efflux transporter	-
SINMEB_RS25020	1587120..1588013	+	894	WP_010967943.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1616209	1616209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6497.32 Da
• Isoelectric Point: 5.7733

>T26514 WP_010967939.1 NC_017324:c1583363-1583190 [Sinorhizobium meliloti BL225C]
MKLVWARYALDDRDAIFSYIERENPRAAVHVDEEVVSAGRPLDFPESRRPGRIAGTP

Nucleotide

• Length: 174 bp

>T26514 NC_017324:c1583363-1583190 [Sinorhizobium meliloti BL225C]
ATGAAGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACGCCATCTTCAGCTACATCGAACGAGAGAATCCGAGGGC
GGCGGTCCACGTTGACGAGGAGGTCGTCAGCGCTGGTCGTCCGCTGGACTTTCCCGAAAGCCGCCGCCCTGGCCGGATTG
CCGGAACTCCATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12053.69 Da
• Isoelectric Point: 11.1401

>AT26514 WP_017268224.1 NC_017324:c1583689-1583360 [Sinorhizobium meliloti BL225C]
VSERLLLIALLALGPFRASQRHTGRPALLRRGPQSAAGAVEEHFIQLRAPLWLITLGGFSHREAHDAWFRAKVLRALEDT
RPDADDADADAHFQERRAAALRKAAAGDR

Nucleotide

• Length: 330 bp

>AT26514 NC_017324:c1583689-1583360 [Sinorhizobium meliloti BL225C]
GTGAGCGAGCGCCTTCTGCTCATCGCCCTCCTGGCCCTCGGTCCGTTCCGAGCCAGCCAGCGGCATACCGGTCGTCCTGC
TCTGCTACGGCGTGGCCCCCAATCGGCCGCCGGCGCGGTCGAGGAGCACTTCATCCAACTCCGCGCTCCGCTGTGGCTAA
TCACGCTCGGCGGTTTCAGCCACAGAGAGGCTCACGACGCTTGGTTCCGCGCCAAAGTTTTGCGGGCACTCGAGGACACT
CGACCCGATGCGGACGACGCCGACGCCGATGCGCACTTTCAAGAGCGCCGCGCCGCAGCTTTGCGCAAGGCTGCGGCGGG
TGATCGATGA

Structures

Toxin

• AlphaFold DB: Q92YA6