Detailed information of TA system TA026513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1046419..1047104
• Replicon: plasmid pSINMEB01
• Accession: NC_017324
• Organism: Sinorhizobium meliloti BL225C

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SINMEB_RS22385
• Protein ID: WP_017263896.1
• Coordinates: 1046419..1046838 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XET8
• Locus tag: SINMEB_RS22390
• Protein ID: WP_013845417.1
• Coordinates: 1046835..1047104 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINMEB_RS22360	1042108..1043481	-	1374	WP_014528618.1	ISNCY family transposase	-
SINMEB_RS22365	1043607..1044608	-	1002	WP_014528068.1	IS30 family transposase	-
SINMEB_RS22370	1044868..1045248	-	381	WP_003526626.1	transcriptional regulator	-
SINMEB_RS22375	1045266..1045577	-	312	WP_014528619.1	type II toxin-antitoxin system HigB family toxin	-
SINMEB_RS22380	1045852..1046265	+	414	WP_014528620.1	hypothetical protein	-
SINMEB_RS22385	1046419..1046838	-	420	WP_017263896.1	type II toxin-antitoxin system VapC family toxin	Toxin
SINMEB_RS22390	1046835..1047104	-	270	WP_013845417.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SINMEB_RS37645	1047242..1047593	+	352	Protein_1015	LysR family transcriptional regulator	-
SINMEB_RS22395	1047704..1048579	-	876	WP_013845418.1	LysR family transcriptional regulator	-
SINMEB_RS22405	1049559..1050482	-	924	WP_014528622.1	LysR family transcriptional regulator	-
SINMEB_RS22410	1050599..1051468	+	870	WP_014528623.1	aldo/keto reductase family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1616209	1616209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.00 Da
• Isoelectric Point: 4.8722

>T26513 WP_017263896.1 NC_017324:c1046838-1046419 [Sinorhizobium meliloti BL225C]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSG

Nucleotide

• Length: 420 bp

>T26513 NC_017324:c1046838-1046419 [Sinorhizobium meliloti BL225C]
GTGAGCAGGCTCTACATGCTGGACACCAATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CATCGAAACTACTGGCGCAGATCGAGACCATTCTTGGAAGTATGCAAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCCGCTGGTAAGCCCATCGGCCCGAATGACCTGTTCATCGCCGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACGGCCAACAGTAGCGAGTTCACACGCGTTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTCCTTCGGGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10203.67 Da
• Isoelectric Point: 4.4798

>AT26513 WP_013845417.1 NC_017324:c1047104-1046835 [Sinorhizobium meliloti BL225C]
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT26513 NC_017324:c1047104-1046835 [Sinorhizobium meliloti BL225C]
ATGCCCGAACTCCGACACAACGAACCAACACCGCCAAGGGAAGCTAAGCTCTTCCGAAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCCGGCGATCGGGTGATGATCCATCGGGACGGAGATCGGCTGATAATAGAGCCGGTGC
TCCGCAAGAACTTGCTGGAGGTGCTGGCTGGTTTGGAGCCGCTCGGGTCTGAAGATGAGTTCCCCGACGTTGACGAAACC
CTGCTGCCGGTGAAGGAAATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A844A3Y5