Detailed information of TA system TA026512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 594469..595067
• Replicon: plasmid pSINMEB01
• Accession: NC_017324
• Organism: Sinorhizobium meliloti BL225C

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0E0UKM8
• Locus tag: SINMEB_RS20270
• Protein ID: WP_014528329.1
• Coordinates: 594469..594762 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q930F1
• Locus tag: SINMEB_RS20275
• Protein ID: WP_010967242.1
• Coordinates: 594759..595067 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINMEB_RS34235	590239..590484	-	246	WP_014528323.1	membrane protein	-
SINMEB_RS20250	591172..591603	-	432	WP_014528325.1	class I SAM-dependent methyltransferase	-
SINMEB_RS20255	591655..592137	-	483	WP_014528326.1	Lrp/AsnC family transcriptional regulator	-
SINMEB_RS20260	592177..592590	+	414	WP_014528327.1	nuclear transport factor 2 family protein	-
SINMEB_RS20265	593008..594207	+	1200	WP_014528328.1	NAD-dependent formate dehydrogenase	-
SINMEB_RS20270	594469..594762	-	294	WP_014528329.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
SINMEB_RS20275	594759..595067	-	309	WP_010967242.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SINMEB_RS34250	595314..595564	+	251	Protein_579	YecA family protein	-
SINMEB_RS20280	595617..596075	-	459	WP_014528330.1	SRPBCC domain-containing protein	-
SINMEB_RS20285	596257..597198	+	942	WP_014528331.1	helix-turn-helix domain-containing protein	-
SINMEB_RS20290	597310..597930	+	621	WP_014528332.1	glutathione S-transferase C-terminal domain-containing protein	-
SINMEB_RS20295	597989..598378	+	390	WP_014528333.1	hypothetical protein	-
SINMEB_RS20300	598486..598914	+	429	WP_017267827.1	VOC family protein	-
SINMEB_RS34255	598946..599200	-	255	Protein_585	nuclear transport factor 2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1616209	1616209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11292.96 Da
• Isoelectric Point: 6.4734

>T26512 WP_014528329.1 NC_017324:c594762-594469 [Sinorhizobium meliloti BL225C]
MRLVWARYALDDRDTIFSYIERENPRAAVHVDEEIVSAVRRLLDFPESGRPGRIAATRELVIPRTPYIAAYMVMEDRIRI
LRVLHGAQKWPSELDDG

Nucleotide

• Length: 294 bp

>T26512 NC_017324:c594762-594469 [Sinorhizobium meliloti BL225C]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGCAACTCGAGAGCTTGTTATCCCTCGGACCCCCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATT
CTGCGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11210.58 Da
• Isoelectric Point: 5.6604

>AT26512 WP_010967242.1 NC_017324:c595067-594759 [Sinorhizobium meliloti BL225C]
MASNALVQTRIDAEVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELFSHSEAHDAWFRAKVLRALEDTRPDVDDA
DADAHFRERRAAALRKAAAGDR

Nucleotide

• Length: 309 bp

>AT26512 NC_017324:c595067-594759 [Sinorhizobium meliloti BL225C]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA

Structures

Toxin

• AlphaFold DB: A0A0E0UKM8

Antitoxin

• AlphaFold DB: Q930F1