Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2777753..2778405 Replicon chromosome
Accession NZ_CP112997
Organism Mycobacterium tuberculosis variant bovis strain 2016/0386

Toxin (Protein)


Gene name vapC Uniprot ID G0TPX1
Locus tag ORO20_RS13035 Protein ID WP_003412752.1
Coordinates 2777980..2778405 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag ORO20_RS13030 Protein ID WP_003412749.1
Coordinates 2777753..2777974 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORO20_RS13015 (ORO20_13015) 2775993..2776199 - 207 WP_003899347.1 hypothetical protein -
ORO20_RS13020 (ORO20_13020) 2776335..2776958 + 624 WP_003900858.1 TIGR00725 family protein -
ORO20_RS13025 (ORO20_13025) 2776948..2777700 + 753 WP_003901416.1 hypothetical protein -
ORO20_RS13030 (ORO20_13030) 2777753..2777974 + 222 WP_003412749.1 antitoxin Antitoxin
ORO20_RS13035 (ORO20_13035) 2777980..2778405 + 426 WP_003412752.1 type II toxin-antitoxin system VapC family toxin Toxin
ORO20_RS13040 (ORO20_13040) 2778428..2779609 - 1182 WP_268141902.1 dihydrolipoamide acetyltransferase family protein -
ORO20_RS13045 (ORO20_13045) 2779606..2780652 - 1047 WP_010950730.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
ORO20_RS13050 (ORO20_13050) 2780663..2781766 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
ORO20_RS13055 (ORO20_13055) 2782025..2782846 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
ORO20_RS13060 (ORO20_13060) 2782843..2783400 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15392.89 Da        Isoelectric Point: 9.2386

>T265080 WP_003412752.1 NZ_CP112997:2777980-2778405 [Mycobacterium tuberculosis variant bovis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRVSTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T265080 NZ_CP112997:2777980-2778405 [Mycobacterium tuberculosis variant bovis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT265080 WP_003412749.1 NZ_CP112997:2777753-2777974 [Mycobacterium tuberculosis variant bovis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT265080 NZ_CP112997:2777753-2777974 [Mycobacterium tuberculosis variant bovis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References