Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3237791..3238452 Replicon chromosome
Accession NC_017322
Organism Sinorhizobium meliloti BL225C

Toxin (Protein)


Gene name vapC Uniprot ID Q92KY5
Locus tag SINMEB_RS15370 Protein ID WP_010970565.1
Coordinates 3237791..3238195 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q92KY4
Locus tag SINMEB_RS15375 Protein ID WP_010970566.1
Coordinates 3238192..3238452 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SINMEB_RS15355 3233334..3234446 + 1113 WP_003535893.1 3-isopropylmalate dehydrogenase -
SINMEB_RS15360 3234575..3235282 + 708 WP_010970563.1 phosphatase PAP2 family protein -
SINMEB_RS15365 3235383..3237272 - 1890 WP_014527006.1 MHS family MFS transporter -
SINMEB_RS15370 3237791..3238195 - 405 WP_010970565.1 type II toxin-antitoxin system VapC family toxin Toxin
SINMEB_RS15375 3238192..3238452 - 261 WP_010970566.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
SINMEB_RS33700 3238581..3238751 + 171 WP_010970567.1 hypothetical protein -
SINMEB_RS15380 3238865..3239899 + 1035 WP_003531790.1 aspartate-semialdehyde dehydrogenase -
SINMEB_RS15385 3240072..3240893 + 822 WP_010970568.1 lytic transglycosylase family protein -
SINMEB_RS15390 3241240..3241923 - 684 WP_010970569.1 carbonic anhydrase -
SINMEB_RS15395 3242097..3242978 - 882 WP_010970570.1 pyridoxal kinase PdxY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(8-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14507.71 Da        Isoelectric Point: 6.7257

>T26506 WP_010970565.1 NC_017322:c3238195-3237791 [Sinorhizobium meliloti BL225C]
VISHILDTNAVIALIGRKSDALVTRVLHSPQGIIGLPSVVAYELYFGAQKSAKAQHNLETLRLLMADFPILDFDRNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLRVEDWSL

Download         Length: 405 bp

>T26506 NC_017322:c3238195-3237791 [Sinorhizobium meliloti BL225C]
GTGATCTCCCATATTCTCGACACCAACGCCGTCATCGCTTTGATCGGGCGCAAATCGGATGCATTGGTCACCCGTGTACT
CCATAGCCCTCAGGGCATTATCGGGCTCCCGTCGGTCGTCGCGTATGAACTCTATTTTGGAGCGCAGAAAAGCGCGAAGG
CCCAACACAATCTCGAAACCCTGCGTTTGTTGATGGCGGATTTTCCGATCCTCGACTTCGACCGGAACGATGCCTTTGTC
GCAGGCGAGATCCGGGCCGCCCTTGCCGCGAAGGGTACGCCCATCGGACCTTATGACGTGCTGATTGCGGGACAGGCCAA
AGCCCGTGGATTGACGCTCGTCACCAACAATGTCGGCGAGTTCAATAGGGTTGAGAACCTGCGCGTGGAGGATTGGTCGC
TCTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9903.16 Da        Isoelectric Point: 5.1253

>AT26506 WP_010970566.1 NC_017322:c3238452-3238192 [Sinorhizobium meliloti BL225C]
MPHLARVFQSGNSQAVRLPKEFRFNVDRVEITQEGDALILRPHVEQGEQWSSLKAALARGMSEDFMMCGREQPEQQDRSE
LDAVFR

Download         Length: 261 bp

>AT26506 NC_017322:c3238452-3238192 [Sinorhizobium meliloti BL225C]
ATGCCCCATCTTGCACGGGTGTTCCAGTCCGGAAATTCGCAGGCCGTCCGCCTGCCTAAGGAATTCCGCTTCAACGTCGA
TCGCGTCGAAATCACGCAGGAAGGCGACGCGCTCATTCTCCGCCCGCACGTCGAGCAAGGCGAGCAGTGGTCATCGCTGA
AGGCGGCACTTGCCCGTGGCATGAGTGAAGACTTCATGATGTGCGGGCGCGAGCAGCCGGAACAGCAGGATCGGTCGGAA
CTCGACGCGGTATTTCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92KY5

Antitoxin

Source ID Structure
AlphaFold DB Q92KY4

References