Detailed information of TA system TA026505

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2600956..2601587
• Replicon: chromosome
• Accession: NC_017322
• Organism: Sinorhizobium meliloti BL225C

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SINMEB_RS12525
• Protein ID: WP_013844828.1
• Coordinates: 2600956..2601354 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92MD9
• Locus tag: SINMEB_RS12530
• Protein ID: WP_010970129.1
• Coordinates: 2601354..2601587 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINMEB_RS12490	2596038..2596340	-	303	WP_010970122.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SINMEB_RS12495	2596337..2596612	-	276	WP_010970123.1	type II toxin-antitoxin system ParD family antitoxin	-
SINMEB_RS33590	2596898..2597047	+	150	WP_010970124.1	hypothetical protein	-
SINMEB_RS12505	2597044..2597979	+	936	WP_014526971.1	site-specific tyrosine recombinase XerD	-
SINMEB_RS12510	2598043..2598996	+	954	WP_003527479.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
SINMEB_RS12515	2599241..2600683	+	1443	WP_010970126.1	murein L,D-transpeptidase	-
SINMEB_RS12520	2600683..2600922	+	240	WP_010970127.1	sulfurtransferase TusA family protein	-
SINMEB_RS12525	2600956..2601354	-	399	WP_013844828.1	type II toxin-antitoxin system toxin VapC	Toxin
SINMEB_RS12530	2601354..2601587	-	234	WP_010970129.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SINMEB_RS12535	2601696..2602841	-	1146	WP_010970130.1	GTP-binding protein	-
SINMEB_RS12540	2602855..2603937	-	1083	WP_010970131.1	D-alanyl-D-alanine carboxypeptidase	-
SINMEB_RS12545	2604189..2605352	+	1164	WP_010970132.1	amidohydrolase	-
SINMEB_RS12550	2605375..2605839	+	465	WP_010970133.1	Lrp/AsnC ligand binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14829.13 Da
• Isoelectric Point: 6.9581

>T26505 WP_013844828.1 NC_017322:c2601354-2600956 [Sinorhizobium meliloti BL225C]
MLTYMLDTNICIYVMKTYPPAVREKFNGLAEQLCISSITLGELHYGAEKSARRVENLTAIEHFVARLEVLPFADKAAAHY
GQVRAELERTGTPCGPHDMQIGAHARSEGLIVVTNNIREFVRMPGVRVENWL

Nucleotide

• Length: 399 bp

>T26505 NC_017322:c2601354-2600956 [Sinorhizobium meliloti BL225C]
ATGCTCACCTATATGCTCGATACCAATATCTGCATCTATGTGATGAAGACCTATCCGCCGGCCGTGCGCGAAAAGTTCAA
CGGACTGGCGGAGCAGCTTTGCATATCGAGCATCACGCTGGGAGAATTACACTATGGCGCCGAAAAATCGGCCCGGCGCG
TTGAGAACCTTACGGCTATCGAACATTTCGTGGCACGGCTGGAGGTGCTGCCGTTCGCAGACAAGGCAGCGGCCCACTAC
GGGCAGGTTCGGGCTGAACTGGAGCGGACGGGAACGCCATGCGGCCCTCATGACATGCAGATCGGCGCGCATGCGCGCAG
CGAAGGGCTGATTGTCGTGACCAACAATATACGCGAGTTCGTCCGCATGCCGGGTGTAAGGGTCGAGAACTGGCTGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8673.87 Da
• Isoelectric Point: 8.9167

>AT26505 WP_010970129.1 NC_017322:c2601587-2601354 [Sinorhizobium meliloti BL225C]
MASSTVFISNRSQAVRLPKAVAFPEGVHQVDILKIGRSRVIVPQGKRWDDLFLSGPRVSEDFMSERDQPVAETRESF

Nucleotide

• Length: 234 bp

>AT26505 NC_017322:c2601587-2601354 [Sinorhizobium meliloti BL225C]
TTGGCCAGTTCGACCGTATTCATCAGTAACCGTAGCCAGGCGGTGCGGCTGCCAAAGGCCGTCGCCTTTCCCGAGGGCGT
GCATCAGGTCGATATCCTCAAGATCGGCCGCAGCCGCGTGATCGTCCCCCAGGGCAAAAGATGGGACGATCTGTTCCTCA
GCGGGCCGCGCGTCAGCGAGGATTTCATGAGCGAGCGTGATCAGCCGGTGGCGGAAACACGCGAATCCTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q92MD9