Detailed information of TA system TA026504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 2553409..2554175
• Replicon: chromosome
• Accession: NC_017322
• Organism: Sinorhizobium meliloti BL225C

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: H0G6D3
• Locus tag: SINMEB_RS12260
• Protein ID: WP_003533488.1
• Coordinates: 2553693..2554175 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: H0G6D4
• Locus tag: SINMEB_RS12255
• Protein ID: WP_003533490.1
• Coordinates: 2553409..2553693 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINMEB_RS12240	2548879..2550267	+	1389	WP_014526970.1	lipopolysaccharide biosynthesis protein	-
SINMEB_RS12245	2550271..2551542	+	1272	WP_010970087.1	GNAT family N-acetyltransferase	-
SINMEB_RS12250	2551558..2553204	-	1647	WP_010970088.1	AMP-binding protein	-
SINMEB_RS12255	2553409..2553693	+	285	WP_003533490.1	DUF1778 domain-containing protein	Antitoxin
SINMEB_RS12260	2553693..2554175	+	483	WP_003533488.1	GNAT family N-acetyltransferase	Toxin
SINMEB_RS12270	2554640..2555149	+	510	WP_013844819.1	disulfide bond formation protein B	-
SINMEB_RS12275	2555181..2555738	-	558	WP_003533486.1	HNH endonuclease	-
SINMEB_RS12280	2555844..2556491	-	648	WP_010970091.1	DNA-3-methyladenine glycosylase	-
SINMEB_RS12285	2556644..2557540	+	897	WP_010970092.1	tRNA glutamyl-Q(34) synthetase GluQRS	-
SINMEB_RS12290	2557559..2558434	-	876	WP_010970093.1	YihY/virulence factor BrkB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17463.88 Da
• Isoelectric Point: 7.3827

>T26504 WP_003533488.1 NC_017322:2553693-2554175 [Sinorhizobium meliloti BL225C]
MLSAPTPLGEAHDFDLFQSGNDTLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Nucleotide

• Length: 483 bp

>T26504 NC_017322:2553693-2554175 [Sinorhizobium meliloti BL225C]
ATGCTGTCGGCGCCGACGCCTCTTGGTGAAGCCCACGACTTCGATCTCTTCCAGAGCGGCAACGACACGCTCGATGACTG
GCTGCGCCGCCGTGCGCACGCTAATCAGGCCAGTGGCGCGTCGCGCACCTATGTTATCGCTGAAGAATGGCGCGTGGTCG
GCTATTACTGCCTTGCATCCGGTGCCCTCGATCTGGCCGACGCGCCGAGCTCTGTCCGGCGCAACATGCCGGATCCCATC
CCGATGGCGGTGCTCGGTCGCCTTGCTATCGACCGCGACTGGCAGGGCAAAGGGTTGGGCGCGGCGCTATTGCAAGATGC
CGTCCTGCGGTCCTCGCAGGCGGCCGACATCATGGGAATACGGGGCTTGCTCGTGCATGCCATCTCGGGCGAGGCGAAAG
CCTTTTACGAACATTACGGCTTCCAGTGCTCACCCAACCATCCCATGACGCTGGTGTTGTCGCTCAAGGGCAAGCGCCGT
TAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10771.12 Da
• Isoelectric Point: 7.3175

>AT26504 WP_003533490.1 NC_017322:2553409-2553693 [Sinorhizobium meliloti BL225C]
MATKSSTDTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Nucleotide

• Length: 285 bp

>AT26504 NC_017322:2553409-2553693 [Sinorhizobium meliloti BL225C]
ATGGCCACGAAATCGTCGACTGACACCCACGCGAAGCCCGTCAACCTGCGAATCCGAGAGGATATACGCAAGGTGATCGA
TCGCGCCGCGAGCATTCGCGGCAAGACCCGCTCCGATTTTATGATCGACGCAGCCTACCGCGCGGCCGAAGACACGCTGC
TCGATCAGACGCTGGTGCGGGTCGATGAGCACAGCTATCGGCACTACCTAGACATTCTTGACCAGCCGCCAAGTGGCGAC
GGCTTCGAACGCCTGATGAATGCGCCGAAACCCTGGCAGTCCTGA

Structures

Toxin

• AlphaFold DB: H0G6D3

Antitoxin

• AlphaFold DB: H0G6D4