Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2217752..2218338 Replicon chromosome
Accession NZ_CP112996
Organism Mycobacterium tuberculosis variant bovis strain 2018/0565

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag ORO19_RS10385 Protein ID WP_003410010.1
Coordinates 2217752..2218096 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag ORO19_RS10390 Protein ID WP_003410014.1
Coordinates 2218090..2218338 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORO19_RS10350 (ORO19_10350) 2213458..2214057 + 600 WP_003409989.1 L-lysine exporter -
ORO19_RS10355 (ORO19_10355) 2214473..2214901 + 429 WP_003409992.1 cellulose-binding protein -
ORO19_RS10360 (ORO19_10360) 2215127..2215666 + 540 WP_031702531.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
ORO19_RS10365 (ORO19_10365) 2216186..2216746 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
ORO19_RS10370 (ORO19_10370) 2216743..2217084 - 342 WP_003410003.1 DUF2384 domain-containing protein -
ORO19_RS10375 (ORO19_10375) 2217170..2217427 + 258 WP_003410006.1 hypothetical protein -
ORO19_RS10380 (ORO19_10380) 2217328..2217663 - 336 WP_003410009.1 dehydrogenase -
ORO19_RS10385 (ORO19_10385) 2217752..2218096 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
ORO19_RS10390 (ORO19_10390) 2218090..2218338 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
ORO19_RS10395 (ORO19_10395) 2218438..2220753 - 2316 WP_003899120.1 cation transporter ATPase CptG -
ORO19_RS10400 (ORO19_10400) 2220750..2221022 - 273 WP_003410017.1 DUF1490 family protein -
ORO19_RS10405 (ORO19_10405) 2221075..2221431 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
ORO19_RS10410 (ORO19_10410) 2221588..2222355 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T265016 WP_003410010.1 NZ_CP112996:c2218096-2217752 [Mycobacterium tuberculosis variant bovis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT265016 WP_003410014.1 NZ_CP112996:c2218338-2218090 [Mycobacterium tuberculosis variant bovis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References