Detailed information of TA system TA026498

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 245594..246171
• Replicon: chromosome
• Accession: NC_017322
• Organism: Sinorhizobium meliloti BL225C

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q92S20
• Locus tag: SINMEB_RS32975
• Protein ID: WP_010968707.1
• Coordinates: 245594..245857 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q92KN5
• Locus tag: SINMEB_RS01115
• Protein ID: WP_010968708.1
• Coordinates: 245854..246171 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINMEB_RS01075	240923..241363	+	441	WP_010968702.1	DUF2948 family protein	-
SINMEB_RS01080	241428..242732	+	1305	WP_010968703.1	histidinol dehydrogenase	-
SINMEB_RS01085	242729..243205	+	477	WP_010968704.1	UPF0262 family protein	-
SINMEB_RS01090	243219..243698	+	480	WP_010968705.1	low molecular weight phosphatase family protein	-
SINMEB_RS01095	243856..244074	+	219	WP_004435948.1	translation initiation factor IF-1	-
SINMEB_RS01100	244172..244792	+	621	WP_004435951.1	Maf-like protein	-
SINMEB_RS01105	244797..245009	+	213	WP_010968706.1	DNA gyrase inhibitor YacG	-
SINMEB_RS32975	245594..245857	+	264	WP_010968707.1	BrnT family toxin	Toxin
SINMEB_RS01115	245854..246171	+	318	WP_010968708.1	BrnA antitoxin family protein	Antitoxin
SINMEB_RS01120	246374..246838	-	465	WP_013844019.1	type II toxin-antitoxin system HicB family antitoxin	-
SINMEB_RS32980	246835..247023	-	189	WP_013844020.1	hypothetical protein	-
SINMEB_RS01125	247343..248443	+	1101	WP_014526604.1	HPr kinase	-
SINMEB_RS01130	248430..249512	+	1083	WP_010968711.1	nucleotidyltransferase family protein	-
SINMEB_RS01135	249715..250026	+	312	WP_004435964.1	hypothetical protein	-
SINMEB_RS01145	250306..250695	-	390	WP_013844021.1	DUF930 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-103)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.16 Da
• Isoelectric Point: 10.3413

>T26498 WP_010968707.1 NC_017322:245594-245857 [Sinorhizobium meliloti BL225C]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASALIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Nucleotide

• Length: 264 bp

>T26498 NC_017322:245594-245857 [Sinorhizobium meliloti BL225C]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCCTCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12197.83 Da
• Isoelectric Point: 10.0883

>AT26498 WP_010968708.1 NC_017322:245854-246171 [Sinorhizobium meliloti BL225C]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLPPEFFKAMEEHRRSRGRPSLEHPKKQVTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Nucleotide

• Length: 318 bp

>AT26498 NC_017322:245854-246171 [Sinorhizobium meliloti BL225C]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCAAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG

Structures

Toxin

• AlphaFold DB: Q92S20

Antitoxin

• AlphaFold DB: Q92KN5