Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1350250..1351166 Replicon chromosome
Accession NZ_CP112873
Organism Bacillus subtilis strain O4

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag ORF34_RS07120 Protein ID WP_003244695.1
Coordinates 1350420..1351166 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag ORF34_RS07115 Protein ID WP_003232646.1
Coordinates 1350250..1350420 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORF34_RS07080 (1347113) 1347113..1347442 + 330 WP_003232660.1 XkdW family protein -
ORF34_RS07085 (1347439) 1347439..1347603 + 165 WP_003232658.1 XkdX family protein -
ORF34_RS07090 (1347647) 1347647..1348486 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
ORF34_RS07095 (1348539) 1348539..1348808 + 270 WP_003232655.1 hemolysin XhlA family protein -
ORF34_RS07100 (1348821) 1348821..1349084 + 264 WP_003232653.1 phage holin -
ORF34_RS07105 (1349097) 1349097..1349990 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
ORF34_RS07110 (1350027) 1350027..1350164 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
ORF34_RS07115 (1350250) 1350250..1350420 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
ORF34_RS07120 (1350420) 1350420..1351166 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
ORF34_RS07125 (1351276) 1351276..1352277 - 1002 WP_003232642.1 inorganic phosphate transporter -
ORF34_RS07130 (1352290) 1352290..1352907 - 618 WP_003218470.1 DUF47 domain-containing protein -
ORF34_RS07135 (1353183) 1353183..1354499 - 1317 WP_003244819.1 serine/threonine exchanger -
ORF34_RS07140 (1354888) 1354888..1355838 + 951 WP_003245086.1 ring-cleaving dioxygenase -
ORF34_RS07145 (1355939) 1355939..1356085 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T264900 WP_003244695.1 NZ_CP112873:c1351166-1350420 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT264900 WP_003232646.1 NZ_CP112873:c1350420-1350250 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References