Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 520470..521106 Replicon chromosome
Accession NZ_CP112873
Organism Bacillus subtilis strain O4

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ORF34_RS02665 Protein ID WP_003156187.1
Coordinates 520756..521106 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag ORF34_RS02660 Protein ID WP_003225183.1
Coordinates 520470..520751 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORF34_RS02640 (516829) 516829..517428 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
ORF34_RS02645 (517523) 517523..517888 + 366 WP_003234281.1 holo-ACP synthase -
ORF34_RS02650 (518054) 518054..519070 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
ORF34_RS02655 (519185) 519185..520354 + 1170 WP_003234284.1 alanine racemase -
ORF34_RS02660 (520470) 520470..520751 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ORF34_RS02665 (520756) 520756..521106 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ORF34_RS02670 (521221) 521221..522045 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
ORF34_RS02675 (522050) 522050..522415 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
ORF34_RS02680 (522419) 522419..522820 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
ORF34_RS02685 (522832) 522832..523839 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
ORF34_RS02690 (523901) 523901..524230 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
ORF34_RS02695 (524227) 524227..524709 + 483 WP_003234299.1 anti-sigma B factor RsbW -
ORF34_RS02700 (524675) 524675..525463 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
ORF34_RS02705 (525463) 525463..526062 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T264899 WP_003156187.1 NZ_CP112873:520756-521106 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT264899 WP_003225183.1 NZ_CP112873:520470-520751 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References