Detailed information of TA system TA026489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2635352..2635923
• Replicon: chromosome
• Accession: NC_017316
• Organism: Enterococcus faecalis OG1RF

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: OG1RF_RS12725
• Protein ID: WP_002354774.1
• Coordinates: 2635352..2635693 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: OG1RF_RS12730
• Protein ID: WP_002354773.1
• Coordinates: 2635693..2635923 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OG1RF_RS12720	2631367..2634981	-	3615	WP_041161638.1	DNA-directed RNA polymerase subunit beta	-
OG1RF_RS12725	2635352..2635693	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OG1RF_RS12730	2635693..2635923	-	231	WP_002354773.1	hypothetical protein	Antitoxin
OG1RF_RS12735	2636097..2636312	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
OG1RF_RS12740	2636451..2637443	+	993	WP_002413308.1	biotin--[acetyl-CoA-carboxylase] ligase	-
OG1RF_RS12745	2637707..2638345	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
OG1RF_RS12755	2639031..2640647	+	1617	WP_014525060.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T26489 WP_002354774.1 NC_017316:c2635693-2635352 [Enterococcus faecalis OG1RF]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T26489 NC_017316:c2635693-2635352 [Enterococcus faecalis OG1RF]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT26489 WP_002354773.1 NC_017316:c2635923-2635693 [Enterococcus faecalis OG1RF]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT26489 NC_017316:c2635923-2635693 [Enterococcus faecalis OG1RF]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812