Detailed information of TA system TA264880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 99848..100503
• Replicon: chromosome
• Accession: NZ_CP111141
• Organism: Burkholderia pseudomallei strain MSHR1046

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: OSB54_RS13110
• Protein ID: WP_004521942.1
• Coordinates: 99848..100144 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: OSB54_RS13115
• Protein ID: WP_004202809.1
• Coordinates: 100147..100503 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OSB54_RS13080 (OSB54_13080)	95302..95763	-	462	WP_232290550.1	DNA methyltransferase	-
OSB54_RS13085 (OSB54_13085)	95802..95951	-	150	WP_080498701.1	phage tail protein I	-
OSB54_RS13090 (OSB54_13090)	95989..96213	-	225	Protein_89	phage tail protein I	-
OSB54_RS13095 (OSB54_13095)	96206..96550	-	345	WP_004535737.1	hypothetical protein	-
OSB54_RS13100 (OSB54_13100)	97304..97921	-	618	WP_031313497.1	DUF4276 family protein	-
OSB54_RS13105 (OSB54_13105)	97924..99180	-	1257	WP_004552667.1	AAA family ATPase	-
OSB54_RS13110 (OSB54_13110)	99848..100144	+	297	WP_004521942.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OSB54_RS13115 (OSB54_13115)	100147..100503	+	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
OSB54_RS13120 (OSB54_13120)	100547..101602	-	1056	WP_004557286.1	phage portal protein	-
OSB54_RS13125 (OSB54_13125)	101599..101982	-	384	WP_044584020.1	terminase	-
OSB54_RS13135 (OSB54_13135)	102318..102845	-	528	WP_004521941.1	ClpXP protease specificity-enhancing factor	-
OSB54_RS13140 (OSB54_13140)	102915..103526	-	612	WP_004185176.1	glutathione S-transferase N-terminal domain-containing protein	-
OSB54_RS13145 (OSB54_13145)	103619..104377	-	759	WP_004521940.1	cytochrome c1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	93846..101913	8067

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10902.68 Da
• Isoelectric Point: 9.9369

>T264880 WP_004521942.1 NZ_CP111141:99848-100144 [Burkholderia pseudomallei]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRRGKIIVVVLCGGDKS
TQKKDIKLAKQIADELED

Nucleotide

• Length: 297 bp

>T264880 NZ_CP111141:99848-100144 [Burkholderia pseudomallei]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTACTTCGTGCGACGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGACGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT264880 WP_004202809.1 NZ_CP111141:100147-100503 [Burkholderia pseudomallei]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT264880 NZ_CP111141:100147-100503 [Burkholderia pseudomallei]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGAAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3N4G1D3