Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 517991..518627 Replicon chromosome
Accession NZ_CP111073
Organism Bacillus inaquosorum strain BSXE-2102

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag OP701_RS02625 Protein ID WP_003156187.1
Coordinates 518277..518627 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag OP701_RS02620 Protein ID WP_003225183.1
Coordinates 517991..518272 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OP701_RS02600 (OP701_02600) 514352..514951 - 600 WP_003240338.1 rhomboid family intramembrane serine protease -
OP701_RS02605 (OP701_02605) 515046..515411 + 366 WP_003240339.1 holo-ACP synthase -
OP701_RS02610 (OP701_02610) 515576..516592 + 1017 WP_080010740.1 outer membrane lipoprotein carrier protein LolA -
OP701_RS02615 (OP701_02615) 516707..517876 + 1170 WP_060397966.1 alanine racemase -
OP701_RS02620 (OP701_02620) 517991..518272 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
OP701_RS02625 (OP701_02625) 518277..518627 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
OP701_RS02630 (OP701_02630) 518742..519566 + 825 WP_003240350.1 RsbT co-antagonist protein RsbRA -
OP701_RS02635 (OP701_02635) 519571..519936 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
OP701_RS02640 (OP701_02640) 519940..520341 + 402 WP_003240352.1 serine/threonine-protein kinase RsbT -
OP701_RS02645 (OP701_02645) 520353..521360 + 1008 WP_003240354.1 phosphoserine phosphatase RsbU -
OP701_RS02650 (OP701_02650) 521422..521751 + 330 WP_003240357.1 anti-sigma factor antagonist RsbV -
OP701_RS02655 (OP701_02655) 521748..522230 + 483 WP_003240359.1 anti-sigma B factor RsbW -
OP701_RS02660 (OP701_02660) 522196..522984 + 789 WP_003240361.1 RNA polymerase sigma factor SigB -
OP701_RS02665 (OP701_02665) 522984..523583 + 600 WP_060397967.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T264840 WP_003156187.1 NZ_CP111073:518277-518627 [Bacillus inaquosorum]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT264840 WP_003225183.1 NZ_CP111073:517991-518272 [Bacillus inaquosorum]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References