Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 157996..158732 Replicon plasmid p130-1
Accession NZ_CP111009
Organism Escherichia coli strain J53-p130

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag ORI87_RS23625 Protein ID WP_004098919.1
Coordinates 158250..158732 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag ORI87_RS23620 Protein ID WP_004213599.1
Coordinates 157996..158262 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORI87_RS23595 (ORI87_23595) 153228..153785 + 558 WP_004213590.1 recombinase family protein -
ORI87_RS23600 (ORI87_23600) 153788..156757 + 2970 WP_004213592.1 Tn3 family transposase -
ORI87_RS23605 (ORI87_23605) 156799..157293 + 495 WP_004213594.1 hypothetical protein -
ORI87_RS23610 (ORI87_23610) 157354..157557 + 204 WP_004213596.1 HHA domain-containing protein -
ORI87_RS23615 (ORI87_23615) 157571..157801 + 231 WP_004213598.1 hypothetical protein -
ORI87_RS23620 (ORI87_23620) 157996..158262 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
ORI87_RS23625 (ORI87_23625) 158250..158732 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
ORI87_RS23630 (ORI87_23630) 159153..160380 + 1228 Protein_168 IS3 family transposase -
ORI87_RS23635 (ORI87_23635) 160376..160771 - 396 Protein_169 IS3 family transposase -
ORI87_RS23640 (ORI87_23640) 160946..161968 - 1023 WP_131079334.1 porphobilinogen synthase -
ORI87_RS23645 (ORI87_23645) 162032..163378 - 1347 WP_011154555.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA / rmpA2 1..226559 226559
- inside IScluster/Tn - - 153228..160380 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T264798 WP_004098919.1 NZ_CP111009:158250-158732 [Escherichia coli]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT264798 WP_004213599.1 NZ_CP111009:157996-158262 [Escherichia coli]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References